BRENDA - Enzyme Database
show all sequences of 1.3.99.30

A single desaturase gene from red yeast Sporidiobolus pararoseus is responsible for both four- and five-step dehydrogenation of phytoene

Li, C.; Zhang, N.; Song, J.; Wei, N.; Li, B.; Zou, H.; Han, X.; Gene 590, 169-176 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Sporidiobolus pararoseus
Organism
Organism
UniProt
Commentary
Textmining
Sporidiobolus pararoseus
A0A0K0QVD9
bifunctional enzyme, catalyzes both reactions of EC 1.3.99.30 and EC 1.3.99.31
-
Sporidiobolus pararoseus CGMCC 2.5280
A0A0K0QVD9
bifunctional enzyme, catalyzes both reactions of EC 1.3.99.30 and EC 1.3.99.31
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
catalyzes both enzymatic conversion of phytoene to lycopene (fourth step product) and 3,4-didehydrolycopene (fifth step product), reactions of EC 1.3.99.30 and EC 1.3.99.31, respectively
742615
Sporidiobolus pararoseus
?
-
-
-
?
additional information
catalyzes both enzymatic conversion of phytoene to lycopene (fourth step product) and 3,4-didehydrolycopene (fifth step product), reactions of EC 1.3.99.30 and EC 1.3.99.31, respectively
742615
Sporidiobolus pararoseus CGMCC 2.5280
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 62280, calculated
Sporidiobolus pararoseus
Synonyms
Synonyms
Commentary
Organism
CrtI
-
Sporidiobolus pararoseus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Sporidiobolus pararoseus
calculated
-
7.3
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Sporidiobolus pararoseus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
catalyzes both enzymatic conversion of phytoene to lycopene (fourth step product) and 3,4-didehydrolycopene (fifth step product), reactions of EC 1.3.99.30 and EC 1.3.99.31, respectively
742615
Sporidiobolus pararoseus
?
-
-
-
?
additional information
catalyzes both enzymatic conversion of phytoene to lycopene (fourth step product) and 3,4-didehydrolycopene (fifth step product), reactions of EC 1.3.99.30 and EC 1.3.99.31, respectively
742615
Sporidiobolus pararoseus CGMCC 2.5280
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 62280, calculated
Sporidiobolus pararoseus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Sporidiobolus pararoseus
calculated
-
7.3
General Information
General Information
Commentary
Organism
physiological function
the expression product of crtI is essential for phytoene conversion to lycopene and 3,4-didehydrolycopene
Sporidiobolus pararoseus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the expression product of crtI is essential for phytoene conversion to lycopene and 3,4-didehydrolycopene
Sporidiobolus pararoseus
Other publictions for EC 1.3.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742615
Li
A single desaturase gene from ...
Sporidiobolus pararoseus, Sporidiobolus pararoseus CGMCC 2.5280
Gene
590
169-176
2016
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1
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2
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2
1
1
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1
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1
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2
1
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1
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1
1
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698070
Estrada
Novel apocarotenoid intermedia ...
Neurospora crassa
Fungal Genet. Biol.
45
1497-1505
2008
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1
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1
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710923
Verdoes
Metabolic engineering of the c ...
Phaffia rhodozyma, Phaffia rhodozyma CBS 6938
Appl. Environ. Microbiol.
69
3728-3738
2003
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1
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2
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3
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11
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1
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1
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2
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11
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712086
Hausmann
A single five-step desaturase ...
Neurospora crassa
Fungal Genet. Biol.
30
147-153
2000
2
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1
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1
1
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1
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1
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9
1
1
1
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1
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1
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2
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1
1
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1
1
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9
1
1
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1
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