BRENDA - Enzyme Database
show all sequences of 1.3.99.30

A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa

Hausmann, A.; Sandmann, G.; Fungal Genet. Biol. 30, 147-153 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
NAD+
more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa
Neurospora crassa
NADP+
stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa
Neurospora crassa
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Neurospora crassa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
x * 62000, SDS-PAGE
Neurospora crassa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 5 acceptor
Neurospora crassa
the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Neurospora crassa
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor
overall reaction
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-hydroxylycopene + acceptor
-
712086
Neurospora crassa
1-hydroxy-3',4'-didehydrolycopene + reduced acceptor
-
-
-
?
1-hydroxyneurosporene + acceptor
-
712086
Neurospora crassa
1-hydroxylycopene + reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
all-trans-lycopene + acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-zeta-carotene + 3 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
-
-
?
additional information
gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate
712086
Neurospora crassa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 62000, SDS-PAGE
Neurospora crassa
Synonyms
Synonyms
Commentary
Organism
Al-1
-
Neurospora crassa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
addition of FAD results in product formation close to that of the control without additions
Neurospora crassa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
NAD+
more than 2fold stimulation of activity compared to control, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa
Neurospora crassa
NADP+
stimulation is lower than with NAD+, assay with supernatant of Escherichia coli recombinantly expressing phytoene desaturase from Neurospora crassa
Neurospora crassa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
addition of FAD results in product formation close to that of the control without additions
Neurospora crassa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
x * 62000, SDS-PAGE
Neurospora crassa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 5 acceptor
Neurospora crassa
the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-hydroxylycopene + acceptor
-
712086
Neurospora crassa
1-hydroxy-3',4'-didehydrolycopene + reduced acceptor
-
-
-
?
1-hydroxyneurosporene + acceptor
-
712086
Neurospora crassa
1-hydroxylycopene + reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
all-trans-lycopene + acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-zeta-carotene + 3 acceptor
-
712086
Neurospora crassa
all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
-
-
?
additional information
gamma-carotene and 1,1'-dihydroxylycopene are not accepted as substrate
712086
Neurospora crassa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 62000, SDS-PAGE
Neurospora crassa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Neurospora crassa
Other publictions for EC 1.3.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742615
Li
A single desaturase gene from ...
Sporidiobolus pararoseus, Sporidiobolus pararoseus CGMCC 2.5280
Gene
590
169-176
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
698070
Estrada
Novel apocarotenoid intermedia ...
Neurospora crassa
Fungal Genet. Biol.
45
1497-1505
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710923
Verdoes
Metabolic engineering of the c ...
Phaffia rhodozyma, Phaffia rhodozyma CBS 6938
Appl. Environ. Microbiol.
69
3728-3738
2003
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712086
Hausmann
A single five-step desaturase ...
Neurospora crassa
Fungal Genet. Biol.
30
147-153
2000
2
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
9
1
1
1
-
-
-
1
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
9
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-