BRENDA - Enzyme Database
show all sequences of 1.3.99.30

Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Verdoes, J.C.; Sandmann, G.; Visser, H.; Diaz, M.; van Mossel, M.; van Ooyen, A.J.; Appl. Environ. Microbiol. 69, 3728-3738 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
Phaffia rhodozyma
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 5 acceptor
Phaffia rhodozyma
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
15-cis-phytoene + 5 acceptor
Phaffia rhodozyma CBS 6938
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Phaffia rhodozyma
-
-
-
Phaffia rhodozyma CBS 6938
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
15-cis-phytoene + 5 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
all-trans-lycopene + 1 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 1 reduced acceptor
-
-
-
?
all-trans-lycopene + 1 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 1 reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-zeta-carotene + 3 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CrtI
-
Phaffia rhodozyma
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
Phaffia rhodozyma
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
15-cis-phytoene + 5 acceptor
Phaffia rhodozyma
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
15-cis-phytoene + 5 acceptor
Phaffia rhodozyma CBS 6938
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
15-cis-phytoene + 5 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
15-cis-phytoene + 5 acceptor
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
-
-
?
all-trans-lycopene + 1 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 1 reduced acceptor
-
-
-
?
all-trans-lycopene + 1 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 1 reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-neurosporene + 2 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 2 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-phytofluene + 4 acceptor
-
710923
Phaffia rhodozyma CBS 6938
all-trans-3,4-didehydrolycopene + 4 reduced acceptor
-
-
-
?
all-trans-zeta-carotene + 3 acceptor
-
710923
Phaffia rhodozyma
all-trans-3,4-didehydrolycopene + 3 reduced acceptor
-
-
-
?
Other publictions for EC 1.3.99.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742615
Li
A single desaturase gene from ...
Sporidiobolus pararoseus, Sporidiobolus pararoseus CGMCC 2.5280
Gene
590
169-176
2016
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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2
1
1
-
-
-
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-
-
-
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1
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
698070
Estrada
Novel apocarotenoid intermedia ...
Neurospora crassa
Fungal Genet. Biol.
45
1497-1505
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710923
Verdoes
Metabolic engineering of the c ...
Phaffia rhodozyma, Phaffia rhodozyma CBS 6938
Appl. Environ. Microbiol.
69
3728-3738
2003
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
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-
-
-
-
-
11
-
1
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1
-
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-
-
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2
-
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-
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-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712086
Hausmann
A single five-step desaturase ...
Neurospora crassa
Fungal Genet. Biol.
30
147-153
2000
2
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
-
-
9
1
1
1
-
-
-
1
-
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
9
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-