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Literature summary for 1.3.99.29 extracted from

  • Liu, J.; Gerken, H.; Huang, J.; Chen, F.
    Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids (2013), Process Biochem., 48, 788-795.
No PubMed abstract available
Search for more literature for 1.3.99.29

Application

Application Comment Organism
analysis the engineered endogenous CrPDS L505F mutant is a dominant selectable marker for Chlamydomonas reinhardtii and possibly for other green algae Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
gene pds, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3), quantitative real time PCR expression assay Chlamydomonas reinhardtii

Protein Variants

Protein Variants Comment Organism
L505F site-directed mutagenesis, the mutation enhances enzyme desaturation activity by 29% compared to the wild-type enzyme, L505F exhibits a 27.7fold higher resistance to the herbicide norflurazon Chlamydomonas reinhardtii
additional information possibility of engineering the carotenogenic pathway for improved carotenoid production in microalgae, overview Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
4-chloro-5-(methylamino)-2-[3-(trifluoromethyl)phenyl]pyridazin-3(2H)-one
-
 Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + acceptor Chlamydomonas reinhardtii
-
 all-trans-phytofluene + reduced acceptor
-
 ?
15-cis-phytoene + acceptor Chlamydomonas reinhardtii CC-1618
-
 all-trans-phytofluene + reduced acceptor
-
 ?
all-trans-phytofluene + acceptor Chlamydomonas reinhardtii
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
all-trans-phytofluene + acceptor Chlamydomonas reinhardtii CC-1618
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii Q6J213
-
-
Chlamydomonas reinhardtii CC-1618 Q6J213
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + acceptor
-
 Chlamydomonas reinhardtii all-trans-phytofluene + reduced acceptor
-
 ?
15-cis-phytoene + acceptor
-
 Chlamydomonas reinhardtii CC-1618 all-trans-phytofluene + reduced acceptor
-
 ?
15-cis-phytoene + decyl plastoquinone
-
 Chlamydomonas reinhardtii all-trans-phytofluene + decyl plastoquinol
-
 ?
15-cis-phytoene + decyl plastoquinone
-
 Chlamydomonas reinhardtii CC-1618 all-trans-phytofluene + decyl plastoquinol
-
 ?
all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii all-trans-zeta-carotene + reduced acceptor
-
 ?
all-trans-phytofluene + acceptor
-
 Chlamydomonas reinhardtii CC-1618 all-trans-zeta-carotene + reduced acceptor
-
 ?
all-trans-phytofluene + decyl plastoquinone
-
 Chlamydomonas reinhardtii all-trans-zeta-carotene + decyl plastoquinol
-
 ?
all-trans-phytofluene + decyl plastoquinone
-
 Chlamydomonas reinhardtii CC-1618 all-trans-zeta-carotene + decyl plastoquinol
-
 ?

Synonyms

Synonyms Comment Organism
PDS
-
 Chlamydomonas reinhardtii
phytoene desaturase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
decyl-plastoquinone
-
 Chlamydomonas reinhardtii

General Information

General Information Comment Organism
additional information expression of mutant CrPDS-L505F enhances the accumulation of carotenoids, overview Chlamydomonas reinhardtii