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Literature summary for 1.3.99.29 extracted from

  • Li, M.; Gan, Z.; Cui, Y.; Shi, C.; Shi, X.
    Structure and function characterization of the phytoene desaturase related to the lutein biosynthesis in Chlorella protothecoides CS-41 (2013), Mol. Biol. Rep., 40, 3351-3361.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pds, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain JM109 or BL21(DE3), subcloning in Escherichia coli strain DH5alpha Auxenochlorella protothecoides

Protein Variants

Protein Variants Comment Organism
N144D random and site-directed mutagenesis, inactive mutant Auxenochlorella protothecoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, recombinant enzyme, SDS-PAGE Auxenochlorella protothecoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + acceptor Auxenochlorella protothecoides
-
all-trans-phytofluene + reduced acceptor
-
?
15-cis-phytoene + acceptor Auxenochlorella protothecoides CS-41
-
all-trans-phytofluene + reduced acceptor
-
?
all-trans-phytofluene + acceptor Auxenochlorella protothecoides
-
all-trans-zeta-carotene + reduced acceptor
-
?
all-trans-phytofluene + acceptor Auxenochlorella protothecoides CS-41
-
all-trans-zeta-carotene + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Auxenochlorella protothecoides G8Z936 gene pds
-
Auxenochlorella protothecoides CS-41 G8Z936 gene pds
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + acceptor
-
Auxenochlorella protothecoides all-trans-phytofluene + reduced acceptor
-
?
15-cis-phytoene + acceptor
-
Auxenochlorella protothecoides CS-41 all-trans-phytofluene + reduced acceptor
-
?
all-trans-phytofluene + acceptor
-
Auxenochlorella protothecoides all-trans-zeta-carotene + reduced acceptor
-
?
all-trans-phytofluene + acceptor
-
Auxenochlorella protothecoides CS-41 all-trans-zeta-carotene + reduced acceptor
-
?

Subunits

Subunits Comment Organism
? x * 61000, recombinant enzyme, SDS-PAGE Auxenochlorella protothecoides

Synonyms

Synonyms Comment Organism
PDS
-
Auxenochlorella protothecoides
phytoene desaturase
-
Auxenochlorella protothecoides

General Information

General Information Comment Organism
additional information amino acid residue 144 is crutial to the activity of the PDS enzyme Auxenochlorella protothecoides