Cloned (Comment) | Organism |
---|---|
gene pds, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain JM109 or BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Auxenochlorella protothecoides |
Protein Variants | Comment | Organism |
---|---|---|
N144D | random and site-directed mutagenesis, inactive mutant | Auxenochlorella protothecoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, recombinant enzyme, SDS-PAGE | Auxenochlorella protothecoides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | Auxenochlorella protothecoides | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | Auxenochlorella protothecoides CS-41 | - |
all-trans-phytofluene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Auxenochlorella protothecoides | - |
all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | Auxenochlorella protothecoides CS-41 | - |
all-trans-zeta-carotene + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Auxenochlorella protothecoides | G8Z936 | gene pds | - |
Auxenochlorella protothecoides CS-41 | G8Z936 | gene pds | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + acceptor | - |
Auxenochlorella protothecoides | all-trans-phytofluene + reduced acceptor | - |
? | |
15-cis-phytoene + acceptor | - |
Auxenochlorella protothecoides CS-41 | all-trans-phytofluene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Auxenochlorella protothecoides | all-trans-zeta-carotene + reduced acceptor | - |
? | |
all-trans-phytofluene + acceptor | - |
Auxenochlorella protothecoides CS-41 | all-trans-zeta-carotene + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, recombinant enzyme, SDS-PAGE | Auxenochlorella protothecoides |
Synonyms | Comment | Organism |
---|---|---|
PDS | - |
Auxenochlorella protothecoides |
phytoene desaturase | - |
Auxenochlorella protothecoides |
General Information | Comment | Organism |
---|---|---|
additional information | amino acid residue 144 is crutial to the activity of the PDS enzyme | Auxenochlorella protothecoides |