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Literature summary for 1.3.99.28 extracted from
- Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution (2001), J. Biol. Chem., 276, 41161-41164.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F166I | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
| H12Q | mutation has little effect on the product formation | Cereibacter sphaeroides |
| M402T | mutation has a negative effect on percent lycopene production | Cereibacter sphaeroides |
| additional information | directed evolution is used to change the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-producing enzyme, to lycopene. Two generations of random mutagenesis are performed, from which three positive mutants are isolated and sequenced. Site-directed mutagenesis is used to determine the effect of each amino acid change | Cereibacter sphaeroides |
| V68D | mutation changes the product of phytoene desaturation from neurosporene to lycopene | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15-cis-phytoene + 3 acceptor | Cereibacter sphaeroides | the enzyme is involved in carotenoid biosynthesis | all-trans-neurosporene + 3 reduced acceptor | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15-cis-phytoene + 3 acceptor | the enzyme is involved in carotenoid biosynthesis | Cereibacter sphaeroides | all-trans-neurosporene + 3 reduced acceptor | - |
? | |
| phytoene + acceptor | - |
Cereibacter sphaeroides | neurosporene + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CrtI | - |
Cereibacter sphaeroides |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | involved in carotenoid biosynthesis | Cereibacter sphaeroides |
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