Cloned (Comment) | Organism |
---|---|
gene crtI, cloning and expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha | Rubrivivax gelatinosus |
Protein Variants | Comment | Organism |
---|---|---|
L153P/L278P | site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme | Rubrivivax gelatinosus |
L208F | site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme | Rubrivivax gelatinosus |
L208P | site-directed mutagenesis, the mutant shows altered secondary structure compared to the wild-type enzyme | Rubrivivax gelatinosus |
T256M/D355G/L424P | site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme | Rubrivivax gelatinosus |
Y44C/D53G/P134L/V395A | site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme | Rubrivivax gelatinosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
15-cis-phytoene | recombinant mutant L208F, pH 8.0, 28°C | Rubrivivax gelatinosus | |
0.0148 | - |
15-cis-phytoene | recombinant wild-type enzyme, pH 8.0, 28°C | Rubrivivax gelatinosus | |
0.0171 | - |
15-cis-phytoene | recombinant mutant L208P, pH 8.0, 28°C | Rubrivivax gelatinosus | |
0.0331 | - |
all-trans-neurosporene | recombinant wild-type enzyme, pH 8.0, 28°C | Rubrivivax gelatinosus | |
0.0417 | - |
all-trans-neurosporene | recombinant mutant L208P, pH 8.0, 28°C | Rubrivivax gelatinosus | |
0.0658 | - |
all-trans-neurosporene | recombinant mutant L208F, pH 8.0, 28°C | Rubrivivax gelatinosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + FAD | Rubrivivax gelatinosus | - |
all-trans-phytofluene + FADH2 | - |
? | |
15-cis-phytoene + FAD | Rubrivivax gelatinosus CGMCC 6086 | - |
all-trans-phytofluene + FADH2 | - |
? | |
all-trans-neurosporene + FAD | Rubrivivax gelatinosus | - |
lycopene + FADH2 | - |
? | |
all-trans-neurosporene + FAD | Rubrivivax gelatinosus CGMCC 6086 | - |
lycopene + FADH2 | - |
? | |
all-trans-phytofluene + FAD | Rubrivivax gelatinosus | - |
all-trans-zeta-carotene + FADH2 | - |
? | |
all-trans-zeta-carotene + FAD | Rubrivivax gelatinosus | - |
all-trans-neurosporene + FADH2 | - |
? | |
additional information | Rubrivivax gelatinosus | the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview | ? | - |
? | |
additional information | Rubrivivax gelatinosus CGMCC 6086 | the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrivivax gelatinosus | - |
- |
- |
Rubrivivax gelatinosus CGMCC 6086 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + FAD | - |
Rubrivivax gelatinosus | all-trans-phytofluene + FADH2 | - |
? | |
15-cis-phytoene + FAD | - |
Rubrivivax gelatinosus CGMCC 6086 | all-trans-phytofluene + FADH2 | - |
? | |
all-trans-neurosporene + FAD | - |
Rubrivivax gelatinosus | lycopene + FADH2 | - |
? | |
all-trans-neurosporene + FAD | - |
Rubrivivax gelatinosus CGMCC 6086 | lycopene + FADH2 | - |
? | |
all-trans-phytofluene + FAD | - |
Rubrivivax gelatinosus | all-trans-zeta-carotene + FADH2 | - |
? | |
all-trans-zeta-carotene + FAD | - |
Rubrivivax gelatinosus | all-trans-neurosporene + FADH2 | - |
? | |
additional information | the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview | Rubrivivax gelatinosus | ? | - |
? | |
additional information | the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview | Rubrivivax gelatinosus CGMCC 6086 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrtI | - |
Rubrivivax gelatinosus |
phytoene desaturase | - |
Rubrivivax gelatinosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rubrivivax gelatinosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rubrivivax gelatinosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rubrivivax gelatinosus |
General Information | Comment | Organism |
---|---|---|
additional information | Leu208 is exchanged in the neurosporene or lycopene-forming desaturase | Rubrivivax gelatinosus |