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Literature summary for 1.3.99.28 extracted from

  • Stickforth, P.; Sandmann, G.
    Structural and kinetics properties of a mutated phytoene desaturase from Rubrivivax gelatinosus with modified product specificity (2011), Arch. Biochem. Biophys., 505, 118-122.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene crtI, cloning and expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha Rubrivivax gelatinosus

Protein Variants

Protein Variants Comment Organism
L153P/L278P site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme Rubrivivax gelatinosus
L208F site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme Rubrivivax gelatinosus
L208P site-directed mutagenesis, the mutant shows altered secondary structure compared to the wild-type enzyme Rubrivivax gelatinosus
T256M/D355G/L424P site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme Rubrivivax gelatinosus
Y44C/D53G/P134L/V395A site-directed mutagenesis, the mutant shows altered secondary structure and substrate desaturation level with increased lycopene production compared to the wild-type enzyme Rubrivivax gelatinosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
15-cis-phytoene recombinant mutant L208F, pH 8.0, 28°C Rubrivivax gelatinosus
0.0148
-
15-cis-phytoene recombinant wild-type enzyme, pH 8.0, 28°C Rubrivivax gelatinosus
0.0171
-
15-cis-phytoene recombinant mutant L208P, pH 8.0, 28°C Rubrivivax gelatinosus
0.0331
-
all-trans-neurosporene recombinant wild-type enzyme, pH 8.0, 28°C Rubrivivax gelatinosus
0.0417
-
all-trans-neurosporene recombinant mutant L208P, pH 8.0, 28°C Rubrivivax gelatinosus
0.0658
-
all-trans-neurosporene recombinant mutant L208F, pH 8.0, 28°C Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + FAD Rubrivivax gelatinosus
-
all-trans-phytofluene + FADH2
-
?
15-cis-phytoene + FAD Rubrivivax gelatinosus CGMCC 6086
-
all-trans-phytofluene + FADH2
-
?
all-trans-neurosporene + FAD Rubrivivax gelatinosus
-
lycopene + FADH2
-
?
all-trans-neurosporene + FAD Rubrivivax gelatinosus CGMCC 6086
-
lycopene + FADH2
-
?
all-trans-phytofluene + FAD Rubrivivax gelatinosus
-
all-trans-zeta-carotene + FADH2
-
?
all-trans-zeta-carotene + FAD Rubrivivax gelatinosus
-
all-trans-neurosporene + FADH2
-
?
additional information Rubrivivax gelatinosus the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview ?
-
?
additional information Rubrivivax gelatinosus CGMCC 6086 the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Rubrivivax gelatinosus
-
-
-
Rubrivivax gelatinosus CGMCC 6086
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + FAD
-
Rubrivivax gelatinosus all-trans-phytofluene + FADH2
-
?
15-cis-phytoene + FAD
-
Rubrivivax gelatinosus CGMCC 6086 all-trans-phytofluene + FADH2
-
?
all-trans-neurosporene + FAD
-
Rubrivivax gelatinosus lycopene + FADH2
-
?
all-trans-neurosporene + FAD
-
Rubrivivax gelatinosus CGMCC 6086 lycopene + FADH2
-
?
all-trans-phytofluene + FAD
-
Rubrivivax gelatinosus all-trans-zeta-carotene + FADH2
-
?
all-trans-zeta-carotene + FAD
-
Rubrivivax gelatinosus all-trans-neurosporene + FADH2
-
?
additional information the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview Rubrivivax gelatinosus ?
-
?
additional information the phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene, determination of kinetic with the L208 modified desaturase and the specificities for phytoene and neurosporene as substrates, overview Rubrivivax gelatinosus CGMCC 6086 ?
-
?

Synonyms

Synonyms Comment Organism
CrtI
-
Rubrivivax gelatinosus
phytoene desaturase
-
Rubrivivax gelatinosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Rubrivivax gelatinosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rubrivivax gelatinosus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rubrivivax gelatinosus

General Information

General Information Comment Organism
additional information Leu208 is exchanged in the neurosporene or lycopene-forming desaturase Rubrivivax gelatinosus