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show all sequences of 1.3.99.23

The retinol signaling pathway in mouse pluripotent P19 cells

Chen, Y.; Reese, D.H.; J. Cell. Biochem. 112, 2865-2872 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
the mouse pluripotent P-19 cell metabolizes retinol to atRA and thus can be used in a cell-based screen for disruptors of the pathway. The disruption of the pathway is easily detected and quantitated, the P-19 cell provides an in vitro model system for identifying and exploring the mechanism of action of chemicals that interfere with the critical cellular pathway
Mus musculus
Cloned(Commentary)
Commentary
Organism
quantitative real-time PCR expression analysis
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-13,14-dihydroretinol + acceptor
Mus musculus
-
all-trans-retinol + reduced acceptor
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
P-19 cell
pluripotent embryonal carcinoma cells with high enzyme mRNA level, quantitative real-time PCR expression analysis
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-13,14-dihydroretinol + acceptor
-
725598
Mus musculus
all-trans-retinol + reduced acceptor
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
analysis
the mouse pluripotent P-19 cell metabolizes retinol to atRA and thus can be used in a cell-based screen for disruptors of the pathway. The disruption of the pathway is easily detected and quantitated, the P-19 cell provides an in vitro model system for identifying and exploring the mechanism of action of chemicals that interfere with the critical cellular pathway
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
quantitative real-time PCR expression analysis
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-13,14-dihydroretinol + acceptor
Mus musculus
-
all-trans-retinol + reduced acceptor
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
P-19 cell
pluripotent embryonal carcinoma cells with high enzyme mRNA level, quantitative real-time PCR expression analysis
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-13,14-dihydroretinol + acceptor
-
725598
Mus musculus
all-trans-retinol + reduced acceptor
-
-
-
?
Expression
Organism
Commentary
Expression
Mus musculus
effect of retinol on gene expressions in P-19 cells, overview
additional information
General Information
General Information
Commentary
Organism
metabolism
retinol signaling plays an important role in the establishment and maintenance of cellular phenotype in embryonic and adult vertebrate tissues. all-trans-Retinoic acid functions as the activating ligand for a family of ligand-activated transcription factors, the retinoic acid receptors, which form heterodimers with the retinoid X receptors to regulate gene transcription. Through its activation of the receptors, all-trans-retinoic acid regulates the expression of over 500 protein-coding genes
Mus musculus
additional information
the mouse pluripotent P-19 cell metabolizes retinol to all-trans-retinoic acid, analysis of expression of enzymes in the cell involved in the pathway, overview
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
retinol signaling plays an important role in the establishment and maintenance of cellular phenotype in embryonic and adult vertebrate tissues. all-trans-Retinoic acid functions as the activating ligand for a family of ligand-activated transcription factors, the retinoic acid receptors, which form heterodimers with the retinoid X receptors to regulate gene transcription. Through its activation of the receptors, all-trans-retinoic acid regulates the expression of over 500 protein-coding genes
Mus musculus
additional information
the mouse pluripotent P-19 cell metabolizes retinol to all-trans-retinoic acid, analysis of expression of enzymes in the cell involved in the pathway, overview
Mus musculus
Expression (protein specific)
Organism
Commentary
Expression
Mus musculus
effect of retinol on gene expressions in P-19 cells, overview
additional information
Other publictions for EC 1.3.99.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725598
Chen
The retinol signaling pathway ...
Mus musculus
J. Cell. Biochem.
112
2865-2872
2011
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Moise
Increased adiposity in the ret ...
Mus musculus
FASEB J.
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1261-1270
2010
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700301
Moise
Activation of retinoic acid re ...
Homo sapiens
Mol. Pharmacol.
76
1228-1237
2009
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700994
Schupp
Retinol saturase promotes adip ...
Homo sapiens, Mus musculus
Proc. Natl. Acad. Sci. USA
106
1105-1110
2009
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672228
Moise
Specificity of zebrafish retin ...
Danio rerio, Mus musculus
Biochemistry
46
1811-1820
2007
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686872
Nagaoka-Yasuda
An RNAi-based genetic screen f ...
Mus musculus
Free Radic. Biol. Med.
43
781-788
2007
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659486
Moise
Metabolism and transactivation ...
Mus musculus
J. Biol. Chem.
280
27815-27825
2005
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659418
Moise
Identification of all-trans-re ...
Mus musculus
J. Biol. Chem.
279
50230-50242
2004
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