BRENDA - Enzyme Database show
show all sequences of 1.3.99.23

Increased adiposity in the retinol saturase-knockout mouse

Moise, A.R.; Lobo, G.P.; Erokwu, B.; Wilson, D.L.; Peck, D.; Alvarez, S.; Dominguez, M.; Alvarez, R.; Flask, C.A.; de Lera, A.R.; von Lintig, J.; Palczewski, K.; FASEB J. 24, 1261-1270 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
generation of RetSat-/- mice, phenotype in comparison to wild-type mice, overview
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-all-trans-13,14-dihydroretinol + acceptor
Mus musculus
retinol saturase catalyzes the saturation of all-trans-retinol to produce (R)-all-trans-13,14-dihydroretinol
all-trans-retinol + reduced acceptor
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
C57BL/6 mice
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-all-trans-13,14-dihydroretinol + acceptor
retinol saturase catalyzes the saturation of all-trans-retinol to produce (R)-all-trans-13,14-dihydroretinol
711959
Mus musculus
all-trans-retinol + reduced acceptor
-
-
-
r
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
generation of RetSat-/- mice, phenotype in comparison to wild-type mice, overview
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-all-trans-13,14-dihydroretinol + acceptor
Mus musculus
retinol saturase catalyzes the saturation of all-trans-retinol to produce (R)-all-trans-13,14-dihydroretinol
all-trans-retinol + reduced acceptor
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-all-trans-13,14-dihydroretinol + acceptor
retinol saturase catalyzes the saturation of all-trans-retinol to produce (R)-all-trans-13,14-dihydroretinol
711959
Mus musculus
all-trans-retinol + reduced acceptor
-
-
-
r
General Information
General Information
Commentary
Organism
additional information
RetSat deficiency leads to up-regulation of PPARgamma and PPARgamma target expression
Mus musculus
physiological function
RetSat is required for adipocyte differentiation in the 3T3-L1 cell culture model, analysis of the mechanism involved in this putative proadipogenic effect of RetSat, overview. RetSat-null mice have normal levels of retinol and retinyl palmitate in liver, serum, and adipose tissue, but, in contrast to wild-type mice, are deficient in the production of all-trans-13,14-dihydroretinol from dietary vitamin A. Despite accumulating more fat, RetSat-null mice maintained on either low-fat or high-fat diets gain weight and have similar rates of food intake as age- and gender-matched wild-type control littermates, ablation of RetSat does not result in alterations in total body weight gain but could still affect the relative composition and size of adipose stores, phenotype, overview
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
additional information
RetSat deficiency leads to up-regulation of PPARgamma and PPARgamma target expression
Mus musculus
physiological function
RetSat is required for adipocyte differentiation in the 3T3-L1 cell culture model, analysis of the mechanism involved in this putative proadipogenic effect of RetSat, overview. RetSat-null mice have normal levels of retinol and retinyl palmitate in liver, serum, and adipose tissue, but, in contrast to wild-type mice, are deficient in the production of all-trans-13,14-dihydroretinol from dietary vitamin A. Despite accumulating more fat, RetSat-null mice maintained on either low-fat or high-fat diets gain weight and have similar rates of food intake as age- and gender-matched wild-type control littermates, ablation of RetSat does not result in alterations in total body weight gain but could still affect the relative composition and size of adipose stores, phenotype, overview
Mus musculus
Other publictions for EC 1.3.99.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725598
Chen
The retinol signaling pathway ...
Mus musculus
J. Cell. Biochem.
112
2865-2872
2011
-
1
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
711959
Moise
Increased adiposity in the ret ...
Mus musculus
FASEB J.
24
1261-1270
2010
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
700301
Moise
Activation of retinoic acid re ...
Homo sapiens
Mol. Pharmacol.
76
1228-1237
2009
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700994
Schupp
Retinol saturase promotes adip ...
Homo sapiens, Mus musculus
Proc. Natl. Acad. Sci. USA
106
1105-1110
2009
4
2
2
-
4
-
-
-
-
-
2
2
-
3
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
4
2
2
4
-
4
-
-
-
-
-
-
-
2
2
-
-
-
-
-
5
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
4
-
-
672228
Moise
Specificity of zebrafish retin ...
Danio rerio, Mus musculus
Biochemistry
46
1811-1820
2007
-
2
1
-
-
-
-
-
2
-
-
-
-
7
-
-
-
-
-
4
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686872
Nagaoka-Yasuda
An RNAi-based genetic screen f ...
Mus musculus
Free Radic. Biol. Med.
43
781-788
2007
-
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659486
Moise
Metabolism and transactivation ...
Mus musculus
J. Biol. Chem.
280
27815-27825
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659418
Moise
Identification of all-trans-re ...
Mus musculus
J. Biol. Chem.
279
50230-50242
2004
-
-
1
-
-
-
-
-
1
-
-
1
-
5
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-