BRENDA - Enzyme Database show
show all sequences of 1.3.99.23

Retinol saturase promotes adipogenesis and is downregulated in obesity

Schupp, M.; Lefterova, M.I.; Janke, J.; Leitner, K.; Cristancho, A.G.; Mullican, S.E.; Qatanani, M.; Szwergold, N.; Steger, D.J.; Curtin, J.C.; Kim, R.J.; Suh, M.J.; Suh, M.; Albert, M.R.; Engeli, S.; Gudas, L.J.; Lazar, M.A.; Proc. Natl. Acad. Sci. USA 106, 1105-1110 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
peroxisome proliferator activated receptor gamma
PPARgamma
Homo sapiens
peroxisome proliferator activated receptor gamma
PPARgamma
Mus musculus
thiazolidinedione
-
Homo sapiens
thiazolidinedione
-
Mus musculus
Application
Application
Commentary
Organism
medicine
RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state, RetSat is thus a novel target for therapeutic intervention in metabolic disease
Homo sapiens
medicine
RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state, RetSat is thus a novel target for therapeutic intervention in metabolic disease
Mus musculus
Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
-
Mus musculus
Engineering
Amino acid exchange
Commentary
Organism
E34A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Homo sapiens
E34A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Mus musculus
E96A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Homo sapiens
E96A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Mus musculus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
determined by SDS-PAGE and Western Blot analysis
Homo sapiens
60000
-
determined by SDS-PAGE and Western Blot analysis
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-retinol + reduced acceptor
Mus musculus
-
all-trans-13,14-dihydroretinol + acceptor
-
-
?
all-trans-retinol + reduced acceptor
Homo sapiens
-
all-trans-13,14-dihydroretinol + acceptor
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
adipocyte
-
Homo sapiens
-
adipocyte
-
Mus musculus
-
BOSC-23 cell
-
Homo sapiens
-
HEK-293T cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-retinol + reduced acceptor
-
700994
Mus musculus
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
700994
Homo sapiens
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
FAD/NAD dinucleotide binding motif
Homo sapiens
FAD
FAD/NAD dinucleotide binding motif
Mus musculus
NAD+
FAD/NAD dinucleotide binding motif
Homo sapiens
NAD+
FAD/NAD dinucleotide binding motif
Mus musculus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
peroxisome proliferator activated receptor gamma
PPARgamma
Homo sapiens
peroxisome proliferator activated receptor gamma
PPARgamma
Mus musculus
thiazolidinedione
-
Homo sapiens
thiazolidinedione
-
Mus musculus
Application (protein specific)
Application
Commentary
Organism
medicine
RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state, RetSat is thus a novel target for therapeutic intervention in metabolic disease
Homo sapiens
medicine
RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state, RetSat is thus a novel target for therapeutic intervention in metabolic disease
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
-
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
FAD/NAD dinucleotide binding motif
Homo sapiens
FAD
FAD/NAD dinucleotide binding motif
Mus musculus
NAD+
FAD/NAD dinucleotide binding motif
Homo sapiens
NAD+
FAD/NAD dinucleotide binding motif
Mus musculus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E34A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Homo sapiens
E34A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Mus musculus
E96A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Homo sapiens
E96A
mutant, mutation within the highly conserved region of the dinucleotide-binding motif
Mus musculus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
determined by SDS-PAGE and Western Blot analysis
Homo sapiens
60000
-
determined by SDS-PAGE and Western Blot analysis
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
all-trans-retinol + reduced acceptor
Mus musculus
-
all-trans-13,14-dihydroretinol + acceptor
-
-
?
all-trans-retinol + reduced acceptor
Homo sapiens
-
all-trans-13,14-dihydroretinol + acceptor
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
adipocyte
-
Homo sapiens
-
adipocyte
-
Mus musculus
-
BOSC-23 cell
-
Homo sapiens
-
HEK-293T cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-retinol + reduced acceptor
-
700994
Mus musculus
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
700994
Homo sapiens
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
Expression
Organism
Commentary
Expression
Homo sapiens
in adipocytes down-regulated in obesity
down
Mus musculus
in adipocytes down-regulated in obesity
down
Homo sapiens
incubation of mature adipocytes with pioglitazone or the non-thiazolidinedione ligand GW7845 increases RetSat mRNA expression, peroxisome proliferator activated receptor gamma, PPARgamma, is required for RetSat expression in mature adipocytes
up
Mus musculus
incubation of mature adipocytes with pioglitazone or the non-thiazolidinedione ligand GW7845 increases RetSat mRNA expression, peroxisome proliferator activated receptor gamma, PPARgamma, is required for RetSat expression in mature adipocytes
up
Expression (protein specific)
Organism
Commentary
Expression
Homo sapiens
in adipocytes down-regulated in obesity
down
Mus musculus
in adipocytes down-regulated in obesity
down
Homo sapiens
incubation of mature adipocytes with pioglitazone or the non-thiazolidinedione ligand GW7845 increases RetSat mRNA expression, peroxisome proliferator activated receptor gamma, PPARgamma, is required for RetSat expression in mature adipocytes
up
Mus musculus
incubation of mature adipocytes with pioglitazone or the non-thiazolidinedione ligand GW7845 increases RetSat mRNA expression, peroxisome proliferator activated receptor gamma, PPARgamma, is required for RetSat expression in mature adipocytes
up
Other publictions for EC 1.3.99.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725598
Chen
The retinol signaling pathway ...
Mus musculus
J. Cell. Biochem.
112
2865-2872
2011
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1
1
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1
2
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1
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711959
Moise
Increased adiposity in the ret ...
Mus musculus
FASEB J.
24
1261-1270
2010
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1
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2
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700301
Moise
Activation of retinoic acid re ...
Homo sapiens
Mol. Pharmacol.
76
1228-1237
2009
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700994
Schupp
Retinol saturase promotes adip ...
Homo sapiens, Mus musculus
Proc. Natl. Acad. Sci. USA
106
1105-1110
2009
4
2
2
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4
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2
2
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4
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4
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672228
Moise
Specificity of zebrafish retin ...
Danio rerio, Mus musculus
Biochemistry
46
1811-1820
2007
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2
1
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2
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7
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10
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686872
Nagaoka-Yasuda
An RNAi-based genetic screen f ...
Mus musculus
Free Radic. Biol. Med.
43
781-788
2007
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1
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659486
Moise
Metabolism and transactivation ...
Mus musculus
J. Biol. Chem.
280
27815-27825
2005
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659418
Moise
Identification of all-trans-re ...
Mus musculus
J. Biol. Chem.
279
50230-50242
2004
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1
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1
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5
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