BRENDA - Enzyme Database show
show all sequences of 1.3.99.23

Specificity of zebrafish retinol saturase: formation of all-trans-13,14-dihydroretinol and all-trans-7,8-dihydroretinol

Moise, A.R.; Isken, A.; Dominguez, M.; Lera, A.R.; Lintig, J.; Palczewski, K.; Biochemistry 46, 1811-1820 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
conserved function but altered specificity of RetSat in vertebrates
Danio rerio
additional information
conserved function but altered specificity of RetSat in vertebrates
Mus musculus
Cloned(Commentary)
Commentary
Organism
expressed in T-REx-293 cells; into the Xho I site of pCDNA4/TO and expressed in T-REx-293 cells, cloned into the vector pCRII-TOPO
Danio rerio
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Danio rerio
5783
-
membrane
-
Danio rerio
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Danio rerio
-
-
-
Danio rerio
Q5BLE8
-
-
Mus musculus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adult
-
Danio rerio
-
intestine
;
Danio rerio
-
liver
;
Danio rerio
-
additional information
hatchling
Danio rerio
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Mus musculus
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
preferred product
-
-
?
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-13,14-dihydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-3,4-didehydroretinol + reduced acceptor
all-trans-3,4-didehydroretinol is preferentially converted to all-trans-13,14-dihydro-3,4-didehydroretinol
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
-
all-trans-7,8-dihydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Mus musculus
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydroretinol + acceptor
preferred product
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydroretinol + acceptor
all-trans-7,8-dihydroretinol is the preferred product
-
-
?
Application (protein specific)
Application
Commentary
Organism
additional information
conserved function but altered specificity of RetSat in vertebrates
Danio rerio
additional information
conserved function but altered specificity of RetSat in vertebrates
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in T-REx-293 cells
Danio rerio
into the Xho I site of pCDNA4/TO and expressed in T-REx-293 cells, cloned into the vector pCRII-TOPO
Danio rerio
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Danio rerio
5783
-
membrane
-
Danio rerio
16020
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adult
-
Danio rerio
-
intestine
-
Danio rerio
-
liver
-
Danio rerio
-
additional information
hatchling
Danio rerio
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Mus musculus
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
preferred product
-
-
?
all-trans-13,14-didehydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-13,14-dihydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-3,4-didehydroretinol + reduced acceptor
all-trans-3,4-didehydroretinol is preferentially converted to all-trans-13,14-dihydro-3,4-didehydroretinol
672228
Danio rerio
all-trans-13,14-dihydro-3,4-didehydroretinol + acceptor
-
-
-
-
all-trans-7,8-dihydroretinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydro-3,4-didehydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Mus musculus
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-13,14-dihydroretinol + acceptor
-
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydroretinol + acceptor
preferred product
-
-
?
all-trans-retinol + reduced acceptor
-
672228
Danio rerio
all-trans-7,8-dihydroretinol + acceptor
all-trans-7,8-dihydroretinol is the preferred product
-
-
?
Other publictions for EC 1.3.99.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725598
Chen
The retinol signaling pathway ...
Mus musculus
J. Cell. Biochem.
112
2865-2872
2011
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1
1
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1
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3
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1
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1
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1
1
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1
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1
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1
2
2
1
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711959
Moise
Increased adiposity in the ret ...
Mus musculus
FASEB J.
24
1261-1270
2010
-
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1
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1
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3
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1
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1
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1
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1
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2
2
-
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700301
Moise
Activation of retinoic acid re ...
Homo sapiens
Mol. Pharmacol.
76
1228-1237
2009
-
-
-
-
-
-
-
-
-
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1
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2
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3
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1
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1
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3
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1
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700994
Schupp
Retinol saturase promotes adip ...
Homo sapiens, Mus musculus
Proc. Natl. Acad. Sci. USA
106
1105-1110
2009
4
2
2
-
4
-
-
-
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2
2
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3
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5
-
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2
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4
-
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4
2
2
4
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4
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2
2
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5
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2
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-
-
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-
4
-
-
4
-
-
672228
Moise
Specificity of zebrafish retin ...
Danio rerio, Mus musculus
Biochemistry
46
1811-1820
2007
-
2
1
-
-
-
-
-
2
-
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7
-
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4
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10
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2
2
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2
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6
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10
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686872
Nagaoka-Yasuda
An RNAi-based genetic screen f ...
Mus musculus
Free Radic. Biol. Med.
43
781-788
2007
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1
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1
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2
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1
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659486
Moise
Metabolism and transactivation ...
Mus musculus
J. Biol. Chem.
280
27815-27825
2005
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3
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1
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1
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659418
Moise
Identification of all-trans-re ...
Mus musculus
J. Biol. Chem.
279
50230-50242
2004
-
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1
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1
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1
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5
-
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-
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-
3
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1
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1
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1
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1
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3
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1
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