BRENDA - Enzyme Database
show all sequences of 1.3.99.18

XX. Quinaldic acid 4-oxidoreductase from Pseudomonas sp. AK-2 compared to other procaryotic molybdenum-containing hydroxylases

Sauter, M.; Tshisuaka, B.; Fetzner, S.; Lingens, F.; Biol. Chem. Hoppe-Seyler 374, 1037-1046 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Acriflavine
-
Pseudomonas sp.
CoCl2
-
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
KCN
74% reactivation with S2-
Pseudomonas sp.
methanol
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
NaAsO2
-
Pseudomonas sp.
NiCl2
-
Pseudomonas sp.
p-hydroxymercuribenzoate
-
Pseudomonas sp.
Quinacrine
-
Pseudomonas sp.
ZnSO4
-
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Pseudomonas sp.
1.6
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Pseudomonas sp.
Molybdenum
enzyme contains 1.6 g-atom per mol of enzyme
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
300000
-
gel filtration
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
AK-2
-
Pseudomonas sp. AK-2
-
AK-2
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.82
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp.
?
-
-
-
-
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp. AK-2
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
above 60°C
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
-
5 min, 32% loss of activity
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 1.8 mol of FAD per mol of enzyme
Pseudomonas sp.
Pterin molybdenum cofactor
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 1.8 mol of FAD per mol of enzyme
Pseudomonas sp.
Pterin molybdenum cofactor
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Acriflavine
-
Pseudomonas sp.
CoCl2
-
Pseudomonas sp.
Cu2+
-
Pseudomonas sp.
KCN
74% reactivation with S2-
Pseudomonas sp.
methanol
-
Pseudomonas sp.
Mn2+
-
Pseudomonas sp.
NaAsO2
-
Pseudomonas sp.
NiCl2
-
Pseudomonas sp.
p-hydroxymercuribenzoate
-
Pseudomonas sp.
Quinacrine
-
Pseudomonas sp.
ZnSO4
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
Quinaldate
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
Pseudomonas sp.
1.6
-
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
with quinaldate as cosubstrate
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Pseudomonas sp.
Molybdenum
enzyme contains 1.6 g-atom per mol of enzyme
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
32000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
90000
-
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
300000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.82
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp.
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp.
-
quinaldate + acceptor
acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinaldate + acceptor
i.e. quinoline 2-carboxylic acid
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinaldate + acceptor
acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
12575
Pseudomonas sp. AK-2
kynurenate + reduced acceptor
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
12575
Pseudomonas sp. AK-2
-
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp.
?
-
-
-
-
quinoline 8-carboxylic acid + reduced acceptor
-
12575
Pseudomonas sp. AK-2
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
Pseudomonas sp.
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
above 60°C
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
-
5 min, 32% loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pseudomonas sp.
Other publictions for EC 1.3.99.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
12575
Sauter
XX. Quinaldic acid 4-oxidoredu ...
Pseudomonas sp., Pseudomonas sp. AK-2
Biol. Chem. Hoppe-Seyler
374
1037-1046
1993
-
-
-
-
-
-
11
2
-
2
4
-
-
7
-
-
1
-
-
-
1
-
8
1
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
11
-
2
-
2
4
-
-
-
-
1
-
-
1
-
8
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
12576
Fetzner
XVIII. Purification and some p ...
Serratia marcescens 2CC-1, Serratia marcescens
Biol. Chem. Hoppe-Seyler
374
363-376
1993
-
-
-
-
-
1
7
2
-
2
1
2
-
6
-
-
1
-
-
-
1
2
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
2
-
2
1
2
-
-
-
1
-
-
1
2
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-