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Literature summary for 1.3.99.17 extracted from
- Quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation (1995), Eur. J. Biochem., 232, 536-544.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Pseudomonas putida |  |
| Fe | - |
Rhodococcus sp. | |
| Fe | enzyme contains 7.7 mol iron and 7.5 mol acid labile sulfur per mol of enzyme | Comamonas testosteroni | |
| Mo | contains 1.6 mol molybdenum per mol of enzyme | Comamonas testosteroni | |
| Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Pseudomonas putida | |
| Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Comamonas testosteroni | |
| Mo | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Rhodococcus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 32000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 87000 | - |
x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| 360000 | - |
gel filtration | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3-Methyl)-quinoline + acceptor + H2O | Comamonas testosteroni | first step of 3-methylquinoline degradation | ? | - |
? | |
| Quinoline + acceptor + H2O | Comamonas testosteroni | first step of quinoline degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | - |
- |
- |
| Comamonas testosteroni 63 | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida 86 | - |
- |
- |
| Rhodococcus sp. | - |
- |
- |
| Rhodococcus sp. B1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3-methyl)-quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| (3-methyl)-quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| (3-methyl)-quinoline + acceptor | 61% of the activity with quinoline | Comamonas testosteroni | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| (3-methyl)-quinoline + acceptor | 22% of the activity with quinoline | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
| (3-Methyl)-quinoline + acceptor + H2O | first step of 3-methylquinoline degradation | Comamonas testosteroni | ? | - |
? | |
| 4-chloroquinoline + p-iodonitrotetrazolium violet | 5% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 4-chloroquinoline + p-iodonitrotetrazolium violet | 8% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 4-methylquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 4-methylquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 4-methylquinoline + acceptor | 21% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 4-methylquinoline + acceptor | 12% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | 58% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | 25% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | ? | - |
? | |
| 6-hydroxyquinoline + acceptor | 25% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
| 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
| 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | ? | - |
? | |
| 8-chloroquinoline + acceptor | 55% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| 8-chloroquinoline + acceptor | 29% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 8-chloroquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | ? | - |
? | |
| 8-chloroquinoline + acceptor | 29% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
| 8-methylquinoline + acceptor | 32% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
| 8-methylquinoline + acceptor | 54% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| isoquinoline + p-iodonitrotetrazolium violet | 75% of the activity with quinoline | Comamonas testosteroni | ? | - |
? | |
| quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Comamonas testosteroni | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| quinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| Quinoline + acceptor + H2O | first step of quinoline degradation | Comamonas testosteroni | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Pseudomonas putida |
| ? | - |
Rhodococcus sp. |
| ? | x * 22000 + x * 32000 + x * 87000, subunits occuring in the ratio of 1.16:1:0.83, SDS-PAGE | Comamonas testosteroni |
| More | the N-termini of the beta-subunits show striking similarities | Pseudomonas putida |
| More | the N-termini of the beta-subunits show striking similarities | Comamonas testosteroni |
| More | the N-termini of the beta-subunits show striking similarities | Rhodococcus sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas putida | |
| FAD | enzyme contains 1.8 mol FAD per mol of enzyme | Comamonas testosteroni | |
| FAD | enzyme contains 1.8 mol FAD per mol of enzyme | Rhodococcus sp. | |
| molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Pseudomonas putida | |
| molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Comamonas testosteroni | |
| molybdopterin | molybdopterin cytosine dinucleotide is a part of the pterin molybdenum cofactor | Rhodococcus sp. | |
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