Literature summary for 1.3.99.17 extracted from

  • Fetzner, S.
    Bioconversion of pyrimidine by resting cells of quinoline-degrading bacteria (1999), FEMS Microbiol. Lett., 176, 291-299.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of uracil in supernatant of resting cells with a yield of more than 98% Comamonas testosteroni

Inhibitors

Inhibitors Comment Organism Structure
additional information no inbibition by pyrimidine or uracil Comamonas testosteroni

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni
-
growth on quinoline
-
Comamonas testosteroni 63
-
growth on quinoline
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.064
-
substrate pyrimidine, crude extract, pH 7.5, 25°C Comamonas testosteroni
0.0057
-
substrate substrate pyrimidine-4-one, crude extract, pH 7.5, 25°C Comamonas testosteroni
0.237
-
substrate quinoline, crude extract, pH 7.5, 25°C Comamonas testosteroni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: uracil Comamonas testosteroni ?
-
?
additional information no substrate: uracil Comamonas testosteroni 63 ?
-
?
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
?
pyrimidine + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni 63 pyrimidine-4-one + reduced p-iodomitro-tetrazolium violet
-
?
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni ? + reduced p-iodonitro-tetrazolium violet
-
?
pyrimidine-4-one + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni 63 ? + reduced p-iodonitro-tetrazolium violet
-
?
quinoline + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
?
quinoline + p-iodonitro-tetrazolium violet + H2O
-
Comamonas testosteroni 63 isoquinoline-1-one + reduced p-iodonitro-tetrazolium violet
-
?