Literature summary for 1.3.99.17 extracted from

  • Frerichs-Deeken, U.; Goldenstedt, B.; Gahl-Janssen, R.; Kappl, R.; Huttermann, J.; Fetzner, S.
    Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 deltaqor pUF1 and analysis of the Qor proteins (2003), Eur. J. Biochem., 270, 1567-1577.
    View publication on PubMed

Engineering

Protein Variants Comment Organism
additional information enzyme deletion mutant, not able to grow on quinoline Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
Quinoline 25°C, wild-type Pseudomonas putida
0.12
-
Quinoline 25°C, strain 86-1 deltaqor pUF1 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P72222 medium subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline
-
Pseudomonas putida P72223 small subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline
-
Pseudomonas putida P72224 large subunit; strains KT2440 pUF1, 86-1 deltaqor pUF1, growth on quinoline
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity of enzyme purified from strain KT2440 pUF1 is about 10% of the activity of enzyme purified from wild-type Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
quinoline + iodonitrotetrazolium chloride + H2O
-
Pseudomonas putida isoquinoline-1-one + reduced iodonitrotetrazolium chloride
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
74
-
Quinoline 25°C, wild-type Pseudomonas putida
85.4
-
Quinoline 25°C, strain 86-1 deltaqor pUF1 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas putida
molybdopterin cytosine dinucleotide
-
Pseudomonas putida
iron-sulfur centre two distinct 2Fe-2S clusters Pseudomonas putida