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Literature summary for 1.3.99.16 extracted from

  • Lehmann, M.; Tshisuaka, B.; Fetzner, S.; Roger, P.; Lingens, F.
    Purification and characterization of isoquinoline 1-oxidoreductase from Pseudomonas diminuata 7, a novel molybdenum-containing hydroxylase (1994), J. Biol. Chem., 269, 11254-11260.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Brevundimonas diminuta
5-Hydroxyisoquinoline rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide Brevundimonas diminuta
arsenite
-
Brevundimonas diminuta
CN-
-
Brevundimonas diminuta
Isoquinoline slow inhibition in absence of an electron acceptor Brevundimonas diminuta
methanol
-
Brevundimonas diminuta
p-hydroxymercuribenzoate
-
Brevundimonas diminuta

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters Brevundimonas diminuta
Mo enzyme contains 0.85 g-atom of molybdenum per mol of enzyme Brevundimonas diminuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta
80000
-
1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta
93000 95000 gel filtration, analytical ultracentrifugation Brevundimonas diminuta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Isoquinoline + acceptor + H2O Brevundimonas diminuta first enzyme of the isoquinoline catabolism ?
-
?
Isoquinoline + acceptor + H2O Brevundimonas diminuta 7 first enzyme of the isoquinoline catabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-
Brevundimonas diminuta 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevundimonas diminuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.87
-
-
Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxyisoquinoline + acceptor + H2O
-
Brevundimonas diminuta 1,5-dihydroisoquinoline + reduced acceptor
-
?
5-hydroxyisoquinoline + acceptor + H2O
-
Brevundimonas diminuta 7 1,5-dihydroisoquinoline + reduced acceptor
-
?
isoquinoline + acceptor + H2O acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone Brevundimonas diminuta 1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
?
isoquinoline + acceptor + H2O acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone Brevundimonas diminuta 7 1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
?
Isoquinoline + acceptor + H2O first enzyme of the isoquinoline catabolism Brevundimonas diminuta ?
-
?
Isoquinoline + acceptor + H2O first enzyme of the isoquinoline catabolism Brevundimonas diminuta 7 ?
-
?
phthalazine + acceptor + H2O at 89% of the activity compared to isoquinoline Brevundimonas diminuta 1-oxophthalazine + reduced acceptor
-
?
phthalazine + acceptor + H2O at 89% of the activity compared to isoquinoline Brevundimonas diminuta 7 1-oxophthalazine + reduced acceptor
-
?
quinazoline + acceptor + H2O at 45% of the activity compared to isoquinoline Brevundimonas diminuta 4-hydroxyquinazoline + reduced acceptor
-
?
quinazoline + acceptor + H2O at 45% of the activity compared to isoquinoline Brevundimonas diminuta 7 4-hydroxyquinazoline + reduced acceptor
-
?

Subunits

Subunits Comment Organism
dimer 1 * 80000 + 1 * 16000, SDS-PAGE Brevundimonas diminuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
Brevundimonas diminuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
5 min, rapid decrease of activity above Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Brevundimonas diminuta

Cofactor

Cofactor Comment Organism Structure
additional information enzyme contains no FAD Brevundimonas diminuta
Pterin molybdenum cofactor
-
Brevundimonas diminuta