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show all sequences of 1.3.99.16

Purification and characterization of isoquinoline 1-oxidoreductase from Pseudomonas diminuata 7, a novel molybdenum-containing hydroxylase

Lehmann, M.; Tshisuaka, B.; Fetzner, S.; Roger, P.; Lingens, F.; J. Biol. Chem. 269, 11254-11260 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Brevundimonas diminuta
5-Hydroxyisoquinoline
rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide
Brevundimonas diminuta
arsenite
-
Brevundimonas diminuta
CN-
-
Brevundimonas diminuta
Isoquinoline
slow inhibition in absence of an electron acceptor
Brevundimonas diminuta
methanol
-
Brevundimonas diminuta
p-hydroxymercuribenzoate
-
Brevundimonas diminuta
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters
Brevundimonas diminuta
Mo
enzyme contains 0.85 g-atom of molybdenum per mol of enzyme
Brevundimonas diminuta
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
80000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
93000
95000
gel filtration, analytical ultracentrifugation
Brevundimonas diminuta
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Isoquinoline + acceptor + H2O
Brevundimonas diminuta
first enzyme of the isoquinoline catabolism
?
-
-
-
Isoquinoline + acceptor + H2O
Brevundimonas diminuta 7
first enzyme of the isoquinoline catabolism
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brevundimonas diminuta
-
-
-
Brevundimonas diminuta 7
-
-
-
Purification (Commentary)
Commentary
Organism
-
Brevundimonas diminuta
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.87
-
-
Brevundimonas diminuta
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta 7
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta 7
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta
?
-
-
-
-
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta 7
?
-
-
-
-
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
1-oxophthalazine + reduced acceptor
-
-
-
-
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
1-oxophthalazine + reduced acceptor
-
-
-
-
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
60
-
Brevundimonas diminuta
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, rapid decrease of activity above
Brevundimonas diminuta
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Brevundimonas diminuta
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
enzyme contains no FAD
Brevundimonas diminuta
Pterin molybdenum cofactor
-
Brevundimonas diminuta
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
enzyme contains no FAD
Brevundimonas diminuta
Pterin molybdenum cofactor
-
Brevundimonas diminuta
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Brevundimonas diminuta
5-Hydroxyisoquinoline
rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide
Brevundimonas diminuta
arsenite
-
Brevundimonas diminuta
CN-
-
Brevundimonas diminuta
Isoquinoline
slow inhibition in absence of an electron acceptor
Brevundimonas diminuta
methanol
-
Brevundimonas diminuta
p-hydroxymercuribenzoate
-
Brevundimonas diminuta
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters
Brevundimonas diminuta
Mo
enzyme contains 0.85 g-atom of molybdenum per mol of enzyme
Brevundimonas diminuta
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
16000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
80000
-
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
93000
95000
gel filtration, analytical ultracentrifugation
Brevundimonas diminuta
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Isoquinoline + acceptor + H2O
Brevundimonas diminuta
first enzyme of the isoquinoline catabolism
?
-
-
-
Isoquinoline + acceptor + H2O
Brevundimonas diminuta 7
first enzyme of the isoquinoline catabolism
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Brevundimonas diminuta
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
13.87
-
-
Brevundimonas diminuta
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
5-hydroxyisoquinoline + acceptor + H2O
-
12565
Brevundimonas diminuta 7
1,5-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
isoquinoline + acceptor + H2O
acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone
12565
Brevundimonas diminuta 7
1-oxo-1,2-dihydroisoquinoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta
?
-
-
-
-
Isoquinoline + acceptor + H2O
first enzyme of the isoquinoline catabolism
12565
Brevundimonas diminuta 7
?
-
-
-
-
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
1-oxophthalazine + reduced acceptor
-
-
-
-
phthalazine + acceptor + H2O
at 89% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
1-oxophthalazine + reduced acceptor
-
-
-
-
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta
-
quinazoline + acceptor + H2O
at 45% of the activity compared to isoquinoline
12565
Brevundimonas diminuta 7
4-hydroxyquinazoline + reduced acceptor
-
12565
Brevundimonas diminuta 7
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 80000 + 1 * 16000, SDS-PAGE
Brevundimonas diminuta
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
60
-
Brevundimonas diminuta
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, rapid decrease of activity above
Brevundimonas diminuta
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Brevundimonas diminuta
Other publictions for EC 1.3.99.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654134
Boer
On the purification and prelim ...
Brevundimonas diminuta, Brevundimonas diminuta 7
Acta Crystallogr. Sect. F
F61
137-140
2005
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1
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7
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1
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1
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1
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671077
Boer
On the purification and prelim ...
Brevundimonas diminuta, Brevundimonas diminuta 7
Acta Crystallogr. Sect. F
61
137-140
2005
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1
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1
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7
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1
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1
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1
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1
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655605
Israel
Expression of the iorAB genes ...
Brevundimonas diminuta, Brevundimonas diminuta 7
FEMS Microbiol. Lett.
210
123-127
2002
-
-
-
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1
-
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7
-
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2
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2
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1
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-
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-
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12564
Stephan
Quinaldine 4-oxidase from Arth ...
Brevundimonas diminuta
Eur. J. Biochem.
236
155-162
1996
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
3
-
-
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2
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3
-
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-
-
-
-
-
-
-
-
-
-
-
-
12566
Lehmann
Molecular cloning of the isoqu ...
Brevundimonas diminuta 7, Brevundimonas diminuta
J. Biol. Chem.
270
14420-14429
1995
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1
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1
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2
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9
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1
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1
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1
1
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1
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2
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1
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-
-
-
-
-
-
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-
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-
12565
Lehmann
Purification and characterizat ...
Brevundimonas diminuta 7, Brevundimonas diminuta
J. Biol. Chem.
269
11254-11260
1994
-
-
-
-
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7
-
-
2
3
2
-
8
-
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1
-
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-
1
-
10
1
1
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
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7
-
-
-
2
3
2
-
-
-
1
-
-
1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-