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Literature summary for 1.3.98.6 extracted from

  • Kuehner, M.; Schweyen, P.; Hoffmann, M.; Ramos, J.; Reijerse, E.; Lubitz, W.; Broering, M.; Layer, G.
    The auxiliary [4Fe-4S] cluster of the radical SAM heme synthase from Methanosarcina barkeri is involved in electron transfer (2016), Chem. Sci., 7, 4633-4643 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Methanosarcina barkeri

Protein Variants

Protein Variants Comment Organism
C19A/C23A loss of the N-terminal 4Fe-4S-center, mutant is unable to cleave SAM Methanosarcina barkeri
C321A/C324A loss of the C-terminal 4Fe-4S-center, mutant cleaves SAM comparable to the wild-type enzyme Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q46CH7
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-
Methanosarcina barkeri DSM 804 Q46CH7
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrin III + 2 S-adenosyl-L-methionine
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Methanosarcina barkeri ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?
coproporphyrin III + 2 S-adenosyl-L-methionine
-
Methanosarcina barkeri DSM 804 ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?
Cu-coproporphyrin III + 2 S-adenosyl-L-methionine
-
Methanosarcina barkeri ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?
Cu-coproporphyrin III + 2 S-adenosyl-L-methionine
-
Methanosarcina barkeri DSM 804 ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?
Zn-coproporphyrin III + 2 S-adenosyl-L-methionine
-
Methanosarcina barkeri ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?
Zn-coproporphyrin III + 2 S-adenosyl-L-methionine
-
Methanosarcina barkeri DSM 804 ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
?

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center presence of 2 [4Fe-4S]-clusters. The second cluster is not required for substrate binding and formation of the substrate radical but is involved in a late step of AhbD catalysis, most likely in electron transfer from the reaction intermediate to a final electron acceptor. The central iron ion of the porphyrin substrate also participates in the electron transfer from the reaction intermediate to the auxiliary [4Fe-4S] cluster Methanosarcina barkeri