Cloned (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Protein Variants | Comment | Organism |
---|---|---|
C19A/C23A | loss of the N-terminal 4Fe-4S-center, mutant is unable to cleave SAM | Methanosarcina barkeri |
C321A/C324A | loss of the C-terminal 4Fe-4S-center, mutant cleaves SAM comparable to the wild-type enzyme | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q46CH7 | - |
- |
Methanosarcina barkeri DSM 804 | Q46CH7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? | |
coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri DSM 804 | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? | |
Cu-coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? | |
Cu-coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri DSM 804 | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? | |
Zn-coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? | |
Zn-coproporphyrin III + 2 S-adenosyl-L-methionine | - |
Methanosarcina barkeri DSM 804 | ? + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | presence of 2 [4Fe-4S]-clusters. The second cluster is not required for substrate binding and formation of the substrate radical but is involved in a late step of AhbD catalysis, most likely in electron transfer from the reaction intermediate to a final electron acceptor. The central iron ion of the porphyrin substrate also participates in the electron transfer from the reaction intermediate to the auxiliary [4Fe-4S] cluster | Methanosarcina barkeri |