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Literature summary for 1.3.98.5 extracted from

  • Milazzo, L.; Hofbauer, S.; Howes, B.D.; Gabler, T.; Furtmueller, P.G.; Obinger, C.; Smulevich, G.
    Insights into the active site of coproheme decarboxylase from Listeria monocytogenes (2018), Biochemistry, 57, 2044-2057 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.98.5

Cloned(Commentary)

Cloned (Comment) Organism
-
 Listeria monocytogenes serotype 1/2a

Protein Variants

Protein Variants Comment Organism
M149A mutation alters the heme coordination, which becomes a 6-coordinate low spin species with the amide nitrogen atom of the Q187 residue bound to the heme iron. In the mutant, two CO conformers are present corresponding to open (A0) and closed (A1) conformations. Residue M149 is involved in the formation of a covalent bond with a vinyl substituent of heme b at excess of hydrogen peroxide Listeria monocytogenes serotype 1/2a
M149A/Q187A binding of coproheme is significantly decreased. In the absence of the Q187, only the A0 conformer is found Listeria monocytogenes serotype 1/2a
Q187A in the absence of the Q187, only the A0 conformer is found compared to two CO conformers are present corresponding to open (A0) and closed (A1) conformations in mutant M149A Listeria monocytogenes serotype 1/2a

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the ferric form of the wild-type protein is a pentacoordinate quantum mechanically mixed-spin state Listeria monocytogenes serotype 1/2a

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes serotype 1/2a Q8Y5F1
-
-
Listeria monocytogenes serotype 1/2a ATCC BAA-679 Q8Y5F1
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe-coproporphyrin III + 2 H2O2
-
 Listeria monocytogenes serotype 1/2a heme b + 2 CO2 + 4 H2O
-
 ?
Fe-coproporphyrin III + 2 H2O2
-
 Listeria monocytogenes serotype 1/2a ATCC BAA-679 heme b + 2 CO2 + 4 H2O
-
 ?

Synonyms

Synonyms Comment Organism
ChdC
-
 Listeria monocytogenes serotype 1/2a
coproheme decarboxylase
-
 Listeria monocytogenes serotype 1/2a
HemQ
-
 Listeria monocytogenes serotype 1/2a

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 Fe-coproporphyrin III mutant M149A, pH 7, 25°C Listeria monocytogenes serotype 1/2a
0.18
-
 Fe-coproporphyrin III wild-type, pH 7, 25°C Listeria monocytogenes serotype 1/2a