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Literature summary for 1.3.98.3 extracted from

  • Pratibha, P.; Singh, S.K.; Srinivasan, R.; Bhat, S.R.; Sreenivasulu, Y.
    Gametophyte development needs mitochondrial coproporphyrinogen III oxidase function (2017), Plant Physiol., 174, 258-275 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.98.3

Cloned(Commentary)

Cloned (Comment) Organism
gene HEMN1, promoter and transcript analyses of AtHEMN1, recombinant expression of AtHEMN1-GFP fusion protein in mitochondria, quantitative reverse transcription PCR enzyme expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockout by T-DNA insertions in the HEMN1 gene. A 17fold downregulation of AtHEMN1 transcripts occurs in the mutant. The plastidial CPO gene (LIN2) also shows 3.7fold downregulation. The AtHEMN1 mutation prevents the fusion of polar nuclei in the female gametophyte and affects endosperm proliferation. Phenotype, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion recombinant AtHEMN1-GFP fusion protein is targeted to mitochondria Arabidopsis thaliana 5739
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Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FMJ4
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Source Tissue

Source Tissue Comment Organism Textmining
anther
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 Arabidopsis thaliana
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embryo sac
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 Arabidopsis thaliana
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endosperm of developing seeds Arabidopsis thaliana
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flower promoter and transcript analyses indicate that AtHEMN1 is expressed mainly in floral tissues and developing seeds Arabidopsis thaliana
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gametophyte
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 Arabidopsis thaliana
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plant ovule
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 Arabidopsis thaliana
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seed promoter and transcript analyses indicate that AtHEMN1 is expressed mainly in floral tissues and developing seeds Arabidopsis thaliana
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Synonyms

Synonyms Comment Organism
At5g63290 locus name Arabidopsis thaliana
AtHEMN1
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 Arabidopsis thaliana
coproporphyrinogen III oxidase
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 Arabidopsis thaliana
CPO
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 Arabidopsis thaliana
HEMN1
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 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction mutation in Arabidopsis thaliana CPO-coding gene AtHEMN1 adversely affects silique length, ovule number, and seed set. T-DNA insertions in gene HEMN1 cause seed sterility. Athemn1 mutant alleles are transmitted via both male and female gametes, but homozygous mutants are never recovered. Plants carrying Athemn1 mutant alleles show defects in gametophyte development, including nonviable pollen and embryo sacs with unfused polar nuclei. Improper differentiation of the central cell leads to defects in endosperm development. Consequently, embryo development is arrested at the globular stage. Reactive oxygen species Accumulates around the central cell in the female gametophytes. The mutant phenotype is completely rescued by transgenic expression of AtHEMN1. Blockage of tetrapyrrole biosynthesis in the AtHEMN1 mutant leads to increased reactive oxygen species accumulation in anthers and embryo sacs. The accumulated reactive oxygen species disrupt mitochondrial function by altering their membrane polarity in floral tissues. The AtHEMN1 mutation prevents the fusion of polar nuclei in the female gametophyte and affects endosperm proliferation. T-DNA insertion mutant lines of Arabidopsis thaliana show bushy habit and short siliques. Cell specification is not affected in Athemn1-1 mutant embryo sacs. Phenotype, overview Arabidopsis thaliana
metabolism the enzyme plays an important role in the tetrapyrrole biosynthesis pathway in plants, overview Arabidopsis thaliana
physiological function tetrapyrrole biosynthesis is one of the most essential metabolic pathways in almost all organisms. Coproporphyrinogen III oxidase catalyzes the oxidative decarboxylation of coproporphyrinogen III (coprogen) to yield protoporphyrinogen IX (protogen) in the tetrapyrrole biosynthesis pathway Arabidopsis thaliana