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Literature summary for 1.3.98.3 extracted from

  • Abicht, H.K.; Martinez, J.; Layer, G.; Jahn, D.; Solioz, M.
    Lactococcus lactis HemW (HemN) is a haem-binding protein with a putative role in haem trafficking (2012), Biochem. J., 442, 335-343.
    View publication on PubMed
Search for more literature for 1.3.98.3

Cloned(Commentary)

Cloned (Comment) Organism
gene hemW or hemN, recombinant expression in the hemN-deficient Escherichia coli mutant strain JW3838 and in strain BL21(DE3) as His-tagged protein. Lactococcus lactis HemW at high levels does not complement the growth deficit of the Escherichia coli DELTAhemN mutant Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form Lactococcus lactis 5829
-
membrane in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form Lactococcus lactis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a heme containing enzyme, HemN contains a [4Fe-4S] cluster Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactococcus lactis HemW shows no coproporphyrinogen III oxidase activity in vivo or in vitro ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 gene hemW or hemN
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged HemN from Escherichia coli strain BL21(DE3) membranes by nickel affinity chromatography and gel filtration Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HemW shows no coproporphyrinogen III oxidase activity in vivo or in vitro Lactococcus lactis ?
-
 ?

Synonyms

Synonyms Comment Organism
HemW
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
heme in vivo, HemW occurs as a heme-free cytosolic form, as well as a heme-containing membrane-associated form Lactococcus lactis
S-adenosyl-L-methionine
-
 Lactococcus lactis

General Information

General Information Comment Organism
evolution HemW-like proteins form a distinct phylogenetic clade. It contains the four cysteine residues of the radical S-adenosyl-L-methionine enzyme motif of CPDH enzymes, structure comparisons, overview. The fourth cysteine residue of the Fe-S cluster motif of Escherichia coli HemN, CX3CX2CXC, is replaced by phenylalanine in HemW and related proteins Lactococcus lactis
metabolism genes hemH and hemW (hemN) show conjectured functions in heme metabolism Lactococcus lactis
physiological function addition of Lactococcus lactis membranes to heme-containing HemW triggers the release of heme from HemW in vitro. Role of HemW in heme trafficking Lactococcus lactis