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Literature summary for 1.3.98.3 extracted from

  • Yokoyama, K.; Numakura, M.; Kudo, F.; Ohmori, D.; Eguchi, T.
    Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin (2007), J. Am. Chem. Soc., 129, 15147-15155.
    View publication on PubMed
Search for more literature for 1.3.98.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-scyllo-inosamine uncompetitive substrate inhibition Niallia circulans
additional information not inhibited by S-adenosyl-L-methionine Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 2-deoxy-scyllo-inosamine in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C Niallia circulans
0.46
-
 S-adenosyl-L-methionine apparent value, in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-bind resin column chromatography Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-scyllo-inosamine + S-adenosyl-L-methionine 100% activity Niallia circulans 3-amino-2,3-dideoxy-scyllo-inosose + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?
2-deoxystreptamine + S-adenosyl-L-methionine 14.4% activity compared to 2-deoxy-scyllo-inosamine Niallia circulans ? + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?
additional information no activity with 1L-chiro-inositol, muco-inositol, allo-inositol, D-glucose, D-glucosamine, D-xylose, 1D-chiro-inositol, and 2,3-dideoxy-scyllo-inosose Niallia circulans ?
-
 ?
myo-inositol + S-adenosyl-L-methionine 0.9% activity compared to 2-deoxy-scyllo-inosamine Niallia circulans ? + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?
scyllo-inositol + S-adenosyl-L-methionine 3.3% activity compared to 2-deoxy-scyllo-inosamine Niallia circulans ? + CO2 + L-methionine + 5'-deoxyadenosine
-
 ?

Synonyms

Synonyms Comment Organism
BtrN BtrN is an unusual radical SAM dehydrogenase catalyzing the oxidation of the hydroxyl group by a radical mechanism Niallia circulans
oxygen-independent coproporphyrinogen-III oxidase
-
 Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 S-adenosyl-L-methionine apparent value, in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C Niallia circulans
0.038
-
 2-deoxy-scyllo-inosamine in 50 mM HEPES-NaOH (pH 8.0) in the presence of 10 mM sodium dithionite at 28°C Niallia circulans

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center
-
 Niallia circulans