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Literature summary for 1.3.98.3 extracted from

  • Layer, G.; Kervio, E.; Morlock, G.; Heinz, D.W.; Jahn, D.; Retey, J.; Schubert, W.D.
    Structural and functional comparison of HemN to other radical SAM enzymes (2005), Biol. Chem., 386, 971-980.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
under strictly anaerobic conditions, comparison of the crystal structures of the three radical SAM enzymes HemN, BioB and MoaA show that the enzymes are structurally significantly different Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ HemN binds a (4Fe-4S) cluster Escherichia coli

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Cereibacter sphaeroides
-
-
-
Cupriavidus necator
-
-
-
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by a single chromatographic step under anaerobic conditions Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + S-adenosyl-L-methionine
-
Escherichia coli protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?
coproporphyrinogen-III + S-adenosyl-L-methionine
-
Cereibacter sphaeroides protoporphyrinogen IX + CO2 + L-methionine + 5'-deoxyadenosine
-
?

Subunits

Subunits Comment Organism
monomer by crystallographic studies Escherichia coli

Synonyms

Synonyms Comment Organism
HemN
-
Salmonella enterica subsp. enterica serovar Typhimurium
HemN
-
Bacillus subtilis
HemN
-
Escherichia coli
HemN
-
Cereibacter sphaeroides
HemN
-
Cupriavidus necator
oxygen-independent coproporphyrinogen III oxidase
-
Salmonella enterica subsp. enterica serovar Typhimurium
oxygen-independent coproporphyrinogen III oxidase
-
Bacillus subtilis
oxygen-independent coproporphyrinogen III oxidase
-
Escherichia coli
oxygen-independent coproporphyrinogen III oxidase
-
Cereibacter sphaeroides
oxygen-independent coproporphyrinogen III oxidase
-
Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli
S-adenosyl-L-methionine HemN binds two SAM molecules Escherichia coli