Protein Variants | Comment | Organism |
---|---|---|
L71F | reaction is somehow slower than for wild-type, binding of dihydroorotate is much tighter than with wild-type | Lactococcus lactis |
L71F/C130S/V133T | addition of the two residues comprising the conserved proton-transfer network of Class 2 dihydroorotate dehydrogenase from Escherichia coli to the C130S Class 1A enzyme of Lactococcus lacits. Mutation does not did not restore the function of the active site base or rapid flavin reduction. Kd for dihydroorotate is about three times tighter than the wild-type | Lactococcus lactis |
L71F/V133T | reaction is drastically slower forwith wild-type, Kd for dihydroorotate is iabout eight-fold tighter than the wild-type | Lactococcus lactis |
V133T | reaction is somehow slower than for wild-type, binding of dihydroorotate is much weaker than with wild-type | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + fumarate | - |
Lactococcus lactis | orotate + succinate | - |
? |