Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, with 30% PEG 6000, 1 mM dithiothreitol, 0.2 M sodium acetate, and 0.1 M Tris-HCl at pH 8.5 | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
C130A | the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 590 nm | Lactococcus lactis |
C130S | the mutant forms charge-transfer complexes upon binding 3,4-dihydroxybenzoate, but the maximum of the broad charge-transfer bands is shifted to 610 nm | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxybenzoate | - |
Lactococcus lactis | |
3,5-Dihydroxybenzoate | - |
Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + 2,6-dichlorophenolindophenol | - |
Lactococcus lactis | orotate + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class 1A DHOD | - |
Lactococcus lactis |
class 1A dihydroorotate dehydrogenase | - |
Lactococcus lactis |