Application | Comment | Organism |
---|---|---|
medicine | despite their genetic variations, kinetic properties of the three DHODs are conserved, these findings facilitate further exploitation of Trypanosoma cruzi DHOD inhibitors, as chemotherapeutic agents against Chagas' disease | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma cruzi |
genes subcloned int the EcoRI site of pUC18, PCR products cloned in vector pET100/D-TOPO, recombinant DHOD1 and DHOD2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RP | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Orotate | competitively inhibits all three DHOD enzymes to a comparable level | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0243 | - |
dihydroorotate | DHOD at 25°C | Trypanosoma cruzi | |
0.0259 | - |
dihydroorotate | native DHOD at 25°C | Trypanosoma cruzi | |
0.03 | - |
fumarate | 37°C, pH 7.0 | Trypanosoma cruzi | |
0.03 | - |
dihydroorotate | 37°C, pH 7.0 | Trypanosoma cruzi | |
0.03 | - |
dihydroorotate | DHOD1 and DHOD2 at 37°C | Trypanosoma cruzi | |
0.03 | - |
fumarate | DHOD1 at 37°C | Trypanosoma cruzi | |
0.035 | - |
fumarate | 37°C, pH 7.0 | Trypanosoma cruzi | |
0.035 | - |
fumarate | DHOD2 at 37°C | Trypanosoma cruzi | |
0.0439 | - |
fumarate | DHOD at 25°C | Trypanosoma cruzi | |
0.0534 | - |
fumarate | native DHOD at 25°C | Trypanosoma cruzi | |
0.067 | - |
fumarate | 37°C, pH 7.0 | Trypanosoma cruzi | |
0.067 | - |
fumarate | DHOD3 at 37°C | Trypanosoma cruzi | |
0.071 | - |
dihydroorotate | 37°C, pH 7.0 | Trypanosoma cruzi | |
0.071 | - |
dihydroorotate | DHOD3 at 37°C | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34100 | - |
DHOD3, sequence analysis | Trypanosoma cruzi |
34200 | - |
DHOD1 and DHOD2, sequence analysis | Trypanosoma cruzi |
37000 | - |
recombinant DHOD1, DHOD2 and DHOD3 with an N-terminal His6-tag, affinity chromatography | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi | Q4D3W2 | - |
- |
Trypanosoma cruzi | Q4DEJ0 | - |
- |
Trypanosoma cruzi | Q4DGV2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant DHOD1, DHOD2 and DHOD3 with an N-terminal His6-tag, by affinity chromatography | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + ? | - |
? | |
dihydroorotate + fumarate | - |
Trypanosoma cruzi | orotate + reduced fumarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHOD1 | - |
Trypanosoma cruzi |
DHOD2 | - |
Trypanosoma cruzi |
DHOD3 | - |
Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Trypanosoma cruzi |
7 | - |
DHOD1, DHOD2 and DHOD3 | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0156 | - |
Orotate | inhibition of DHOD2 at 25°C, in the presence of 1 mM fumarate | Trypanosoma cruzi | |
0.0205 | - |
Orotate | inhibition of DHOD2 at 25°C, in the presence of 1 mM dihydroorotate | Trypanosoma cruzi | |
0.0424 | - |
Orotate | 37°C, pH 7.0, versus dihydroorotate | Trypanosoma cruzi | |
0.0424 | - |
Orotate | inhibition of DHOD3 at 37°C, in the presence of 1 mM dihydroorotate | Trypanosoma cruzi | |
0.049 | - |
Orotate | 37°C, pH 7.0, versus fumarate | Trypanosoma cruzi | |
0.049 | - |
Orotate | inhibition of DHOD3 at 37°C, in the presence of 1 mM fumarate | Trypanosoma cruzi | |
0.0573 | - |
Orotate | 37°C, pH 7.0, versus dihydroorotate | Trypanosoma cruzi | |
0.0573 | - |
Orotate | inhibition of DHOD1 at 37°C, in the presence of 1 mM dihydroorotate | Trypanosoma cruzi | |
0.0622 | - |
Orotate | 37°C, pH 7.0, versus fumarate | Trypanosoma cruzi | |
0.0622 | - |
Orotate | inhibition of DHOD1 at 37°C, in the presence of 1 mM fumarate | Trypanosoma cruzi | |
0.0714 | - |
Orotate | 37°C, pH 7.0, versus dihydroorotate | Trypanosoma cruzi | |
0.0714 | - |
Orotate | inhibition of DHOD2 at 37°C, in the presence of 1 mM dihydroorotate | Trypanosoma cruzi | |
0.0773 | - |
Orotate | 37°C, pH 7.0, versus fumarate | Trypanosoma cruzi | |
0.0773 | - |
Orotate | inhibition of DHOD2 at 37°C, in the presence of 1 mM fumarate | Trypanosoma cruzi |