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Literature summary for 1.3.98.1 extracted from

  • Jordan, D.B.; Bisaha, J.J.; Picollelli, M.A.
    Catalytic properties of dihydroorotate dehydrogenase from Saccharomyces cerevisiae: studies on pH, alternate substrates, and inhibitors (2000), Arch. Biochem. Biophys., 378, 84-92.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
2-thioorotate orotate analogue, 50% inhibition at 0.018 mM Saccharomyces cerevisiae
5-aminoorotate orotate analogue, 50% inhibition at 1.6 mM Saccharomyces cerevisiae
5-bromoorotate orotate analogue, 50% inhibition at 0.016 mM Saccharomyces cerevisiae
5-Fluoroorotate orotate analogue, 50% inhibition at 0.055 mM Saccharomyces cerevisiae
5-nitroorotate orotate analogue, 50% inhibition at 0.076 mM Saccharomyces cerevisiae
Orotate competitive, with ferricyanide as electron acceptor Saccharomyces cerevisiae
orotate methyl ester orotate analogue, 50% inhibition at 0.71 mM Saccharomyces cerevisiae
oxonic acid orotate analogue, 50% inhibition at 0.0099 mM Saccharomyces cerevisiae
Uracil orotate analogue, 50% inhibition at 2.5 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 S-dihydroorotate with O2 Saccharomyces cerevisiae
0.0084 0.045 S-dihydroorotate with fumarate Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Saccharomyces cerevisiae
72000
-
 sucrose-density-gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + acceptor Saccharomyces cerevisiae fourth step in synthesis of pyrimidine nucleotides orotate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
 family 1 enzyme A
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae
Orange-A column, reverse-affinity step, Mono Q column Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + acceptor preferred electron acceptors in the decreasing order: ferricyanide, 2,6-dichlorophenolindophenol, ubiquinone-0, fumarate and O2 Saccharomyces cerevisiae orotate + reduced acceptor
-
 ?
(S)-dihydroorotate + acceptor fourth step in synthesis of pyrimidine nucleotides Saccharomyces cerevisiae orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor acceptor: 2,6-dichlorophenolindophenol Saccharomyces cerevisiae orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor kinetic isotope effects Saccharomyces cerevisiae orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor acceptor: ferricyanide, coenzyme Q0, fumarate, molecular oxygen Saccharomyces cerevisiae orotate + reduced acceptor
-
 ?
dihydrooxonate + acceptor
-
 Saccharomyces cerevisiae oxonate + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DHOD
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
 S-dihydroorotate with O2 Saccharomyces cerevisiae
23 25 S-dihydroorotate with fumarate, pH 7.5 Saccharomyces cerevisiae
39
-
 dihydrooxonate with ferricyanide Saccharomyces cerevisiae
190
-
 S-dihydroorotate with ferricyanide Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein, 1 FMN per subunit Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0102
-
 Orotate pH 8.0 Saccharomyces cerevisiae