Literature summary for 1.3.8.7 extracted from

Peterson, K.L.; Sergienko, E.E.; Wu, Y.; Kumar, N.R.; Strauss, A.W.; Oleson, A.E.; Muhonen, W.W.; Shabb, J.B.; Srivastava, D.K.
Recombinant human liver medium-chain acyl-CoA dehydrogenase: purification, characterization, and the mechanism of interactions with functionally diverse C8-CoA molecules (1995), Biochemistry, 34, 14942-14953.

Search for more literature for 1.3.8.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens
expression in Escherichia coli	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
2-octynoyl-CoA	-	Homo sapiens
2-octynoyl-CoA	-	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00049	-	indolepropionyl-CoA	oxidase reaction	Sus scrofa
0.00094	-	decanoyl-CoA	-	Sus scrofa
0.0023	-	octanoyl-CoA	-	Sus scrofa
0.0029	-	decanoyl-CoA	-	Homo sapiens
0.003	-	octanoyl-CoA	-	Homo sapiens
0.0036	-	hexanoyl-CoA	-	Sus scrofa
0.0068	-	hexanoyl-CoA	-	Homo sapiens
0.0087	-	indolepropionyl-CoA	-	Homo sapiens
0.0099	-	ferrocenium hexafluorophosphate	substrate indolepropionyl-CoA	Homo sapiens
0.01	-	indolepropionyl-CoA	-	Sus scrofa
0.014	-	furylpropionyl-CoA	-	Sus scrofa
0.0144	-	furylpropionyl-CoA	-	Homo sapiens
0.0426	-	[4-(dimethylamino)phenyl]propionyl-CoA	-	Homo sapiens
0.061	-	[4-(dimethylamino)phenyl]propionyl-CoA	-	Sus scrofa
0.069	-	ferrocenium hexafluorophosphate	substrate octanoyl-CoA	Homo sapiens
0.264	-	butanoyl-CoA	-	Sus scrofa
0.45	-	butanoyl-CoA	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	4 * 43000, recombinant enzyme, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant liver enzyme, DEAE-Sephacel, hydroxyapatite, tandem column-chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.46	-	substrate indolepropionyl-CoA, ferrocenium hexafluorophosphate as electron carrier	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butanoyl-CoA + acceptor	-	Homo sapiens	crotonyl-CoA + reduced acceptor	-	?
butanoyl-CoA + acceptor	-	Sus scrofa	crotonyl-CoA + reduced acceptor	-	?
decanoyl-CoA + ferricenium hexafluorophosphate	-	Sus scrofa	2-decenoyl-CoA + ferrocenium hexafluorophosphate	-	r
decanoyl-CoA + ferrocenium hexafluorophosphate	-	Sus scrofa	trans-dec-2-enoyl-CoA + reduced ferrocenium hexafluorophosphate	-	r
furylpropionyl-CoA + ferricenium hexafluorophosphate	-	Homo sapiens	furylacryloyl-CoA + ferrocenium hexafluorophosphate	-	?
furylpropionyl-CoA + ferricenium hexafluorophosphate	-	Sus scrofa	furylacryloyl-CoA + ferrocenium hexafluorophosphate	-	?
hexanoyl-CoA + ferricenium hexafluorophosphate	-	Sus scrofa	trans-hex-2-enoyl-CoA + ferrocenium hexafluorophosphate	-	r
hexanoyl-CoA + ferrocenium hexafluorophosphate	-	Sus scrofa	trans-hex-2-enoyl-CoA + reduced ferrocenium hexafluorophosphate	-	r
indolepropionyl-CoA + ferricenium hexafluorophosphate	-	Homo sapiens	indoleacryloyl-CoA + ferrocenium hexafluorophosphate	-	?
indolepropionyl-CoA + ferricenium hexafluorophosphate	-	Sus scrofa	indoleacryloyl-CoA + ferrocenium hexafluorophosphate	-	?
indolepropionyl-CoA + O2	very low activity	Homo sapiens	indoleacryloyl-CoA + H2O2	-	?
indolepropionyl-CoA + O2	very low activity	Sus scrofa	indoleacryloyl-CoA + H2O2	-	?
[4-(dimethylamino)phenyl]propionyl-CoA + ferricenium hexafluorophosphate	-	Homo sapiens	4-(dimethylamino)cinnamoyl-CoA + ferrocenium hexafluorophosphate	-	?
[4-(dimethylamino)phenyl]propionyl-CoA + ferricenium hexafluorophosphate	-	Sus scrofa	4-(dimethylamino)cinnamoyl-CoA + ferrocenium hexafluorophosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	-	Sus scrofa
tetramer	4 * 43000, recombinant enzyme, SDS-PAGE	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.016	-	indolepropionyl-CoA	oxidase reaction	Sus scrofa
0.46	-	indolepropionyl-CoA	-	Sus scrofa
0.61	-	[4-(dimethylamino)phenyl]propionyl-CoA	-	Sus scrofa
3.4	-	indolepropionyl-CoA	-	Homo sapiens
4.6	-	[4-(dimethylamino)phenyl]propionyl-CoA	-	Homo sapiens
6.2	-	butanoyl-CoA	-	Sus scrofa
7.4	-	decanoyl-CoA	-	Homo sapiens
8.6	-	decanoyl-CoA	-	Sus scrofa
11.5	-	butanoyl-CoA	-	Homo sapiens
13.2	-	furylpropionyl-CoA	-	Sus scrofa
13.6	-	furylpropionyl-CoA	-	Homo sapiens
14.2	-	hexanoyl-CoA	-	Homo sapiens
19.2	-	octanoyl-CoA	-	Homo sapiens
19.6	-	octanoyl-CoA	-	Sus scrofa
20.6	-	hexanoyl-CoA	-	Sus scrofa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00036	-	2-octynoyl-CoA	competitive vs. indolepropionyl-CoA	Homo sapiens

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Release 2023.1 (January 2023)