Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination annd analysis, phylogenetic tree, expression with a cleavable 6-His fusion tag followed by the human rhinovirus 3C protease-cleavage sequence in Escherichia coli strain BL21 (DE3) | Burkholderia pseudomallei |
Crystallization (Comment) | Organism |
---|---|
purified detagged recombinant apoenzyme without FAD, sitting drop vapor diffusion method, 26 mg/ml protein in 25 mM Tris pH 8.0, 200 mM NaCl, 1% glycerol, and 1 mM TCEP, with reservoir solution consisting of 20% PEG 3000, 100 mM HEPES, 200 mM NaCl, pH 7.5, 16°C, X-ray diffraction structure determination and analysis at 1.99-2.40 A resolution | Burkholderia pseudomallei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + electron transfer flavoprotein | Homo sapiens | - |
(E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? | |
glutaryl-CoA + electron transfer flavoprotein | Burkholderia pseudomallei | - |
(E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? | |
glutaryl-CoA + electron transfer flavoprotein | Burkholderia pseudomallei 1710b | - |
(E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pseudomallei | Q3JP94 | - |
- |
Burkholderia pseudomallei 1710b | Q3JP94 | - |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration | Burkholderia pseudomallei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + electron transfer flavoprotein | - |
Homo sapiens | (E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? | |
glutaryl-CoA + electron transfer flavoprotein | - |
Burkholderia pseudomallei | (E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? | |
glutaryl-CoA + electron transfer flavoprotein | - |
Burkholderia pseudomallei 1710b | (E)-but-2-enoyl-CoA + CO2 + reduced electron transfer flavoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCDH | - |
Homo sapiens |
GCDH | - |
Burkholderia pseudomallei |
General Information | Comment | Organism |
---|---|---|
evolution | glutaryl-CoA dehydrogenase belongs to the acyl-CoA dehydrogenase enzyme family | Homo sapiens |
evolution | glutaryl-CoA dehydrogenase belongs to the acyl-CoA dehydrogenase enzyme family, phylogenetic tree, overview | Burkholderia pseudomallei |
malfunction | defects in glutaryl-CoA dehydrogenase are involved in glutaric acidemia type 1, an inherited metabolic disorder which can cause macrocephaly, muscular rigidity, spastic paralysis and other progressive movement disorders in humans | Homo sapiens |
metabolism | the enzyme catalyzes an intermediate step in the metabolic breakdown of lysine and tryptophan | Homo sapiens |
metabolism | the enzyme catalyzes an intermediate step in the metabolic breakdown of lysine and tryptophan | Burkholderia pseudomallei |
additional information | the apo structure of GCDH from Burkholderia pseudomallei reveals a loss of secondary structure and increased disorder in the cofactor-binding pocket relative to the ternary complex of the highly homologous human GCDH | Burkholderia pseudomallei |