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Literature summary for 1.3.8.5 extracted from

  • Goetzman, E.S.; Mohsen, A.W.; Prasad, K.; Vockley, J.
    Convergent evolution of a 2-methylbutyryl-CoA dehydrogenase from isovaleryl-CoA dehydrogenase in Solanum tuberosum (2005), J. Biol. Chem., 280, 4873-4879.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain XL1Blue Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
2-methylbutanoyl-CoA recombinant enzyme, 32°C Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 50000, about, SDS-PAGE Solanum tuberosum
188000
-
recombinant enzyme, gel filtration Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli to over 90% purity Solanum tuberosum

Reaction

Reaction Comment Organism Reaction ID
2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ the isovaleryl-CoA dehydrogenase 1, i.e. IVD1, EC 1.3.99.10, from Solanum tuberosum shows maximal activity with 2-methylbutyryl-CoA, the binding pockets of 2-methylbutyryl-CoA and isovaleryl-CoA dehydrogenase are nearly identical, the enzyme therefore shall also be classified to EC 1.3.99.12, active site structure, residues Met361, Ala365, Val88, Val92, and Val96 are involved in catalysis Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
Solanum tuberosum
-
tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbutanoyl-CoA + electron transfer flavoprotein
-
Solanum tuberosum 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
?
butyryl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum but-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?
hexanoyl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum hex-2-enoyl-CoA + reduced electron-transfer flavoprotein
-
?
additional information no activity with isovaleryl-CoA Solanum tuberosum ?
-
?
octanoyl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum oct-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?
valeryl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum pent-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, about, SDS-PAGE Solanum tuberosum

Synonyms

Synonyms Comment Organism
2-methylbutyryl-CoA dehydrogenase
-
Solanum tuberosum
2MBCD
-
Solanum tuberosum
SBCAD
-
Solanum tuberosum
short/branched chain acyl-CoA dehydrogenase
-
Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.77
-
2-methylbutanoyl-CoA per enzyme tetramer, recombinant enzyme, 32°C Solanum tuberosum