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Literature summary for 1.3.8.4 extracted from

  • Karim, R.M.
    Identification of isovaleryl-CoA dehydrogenase catalytic residue in Paracoccus denitrificans PD1222 (2020), Plant Arch., 20, 3091-3096 .
No PubMed abstract available
Search for more literature for 1.3.8.4

Cloned(Commentary)

Cloned (Comment) Organism
gene Pden_3633, located on chromosome 2, recombinant expression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Paracoccus denitrificans

Protein Variants

Protein Variants Comment Organism
E246Q site-directed mutagenesis, the recombinant IVDH enzyme mutant is obtained as an apoprotein, the protein is fully reconstituted by incubation with flavin adenine dinucleotide (FAD) at a ratio 1:20 (IVDH: FAD) molar excess. The reconstituted E246Q IVDH has no activity for isovaleryl-CoA. The mutant IVDH is unable to form charge transfer complex as a result of altering catalytic residue E246 Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovaleryl-CoA + electron-transfer flavoprotein Paracoccus denitrificans
-
 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
isovaleryl-CoA + electron-transfer flavoprotein Paracoccus denitrificans Pd1222
-
 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans A1B856
-
-
Paracoccus denitrificans Pd1222 A1B856
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography Paracoccus denitrificans

Renatured (Commentary)

Renatured (Comment) Organism
the recombinant IVDH enzyme mutant E246Q is obtained as an apoprotein, the protein is fully reconstituted to the holoprotein by incubation with flavin adenine dinucleotide (FAD) at a ratio 1:20 (IVDH: FAD) molar excess. The reconstituted E246Q IVDH has no activity for isovaleryl-CoA Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isovaleryl-CoA + electron-transfer flavoprotein
-
 Paracoccus denitrificans 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
isovaleryl-CoA + electron-transfer flavoprotein
-
 Paracoccus denitrificans Pd1222 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?

Synonyms

Synonyms Comment Organism
IVDH
-
 Paracoccus denitrificans
Pden_3633
-
 Paracoccus denitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
electron transferring flavoprotein ETF Paracoccus denitrificans

General Information

General Information Comment Organism
additional information residue E246 is the predicted active site catalytic residue of the enzyme Paracoccus denitrificans