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Literature summary for 1.3.8.4 extracted from

  • Liu, X.; Wu, L.; Deng, G.; Chen, G.; Li, N.; Chu, X.; Li, D.
    Comparative studies of acyl-CoA dehydrogenases for monomethyl branched chain substrates in amino acid metabolism (2013), Bioorg. Chem., 47, 1-8.
    View publication on PubMed
Search for more literature for 1.3.8.4

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and comparison, expression in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E254G site-directed mutagenesis, inactive mutant Rattus norvegicus
E254G/G375E site-directed mutagenesis, shows no activity with (S)-2-methylbutyryl-CoA in contrast to the wild-type enzyme, reduced activity compared to the wild-type enzyme Rattus norvegicus
G375E site-directed mutagenesis, reduced activity compared to the wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 (S)-2-methylbutyryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
0.0029
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
0.0031
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
0.0035
-
 (S)-2-methylbutyryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
0.0038
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovaleryl-CoA + electron-transfer flavoprotein Rattus norvegicus
-
 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
additional information Rattus norvegicus the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P12007
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-methylbutyryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus 2-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
butyryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus crotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
hexanoyl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus hex-2-enoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
isovaleryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
additional information the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives Rattus norvegicus ?
-
 ?
additional information broader substrate specificity, overview. The enzyme is also active with octanoyl-CoA, decanoyl-CoA, and dodecanoyl-CoA. No activity with isobutyryl-CoA and myristoyl-CoA, with DCIPIP and phenazine methosulfate as second electron acceptors Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
IVD
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
0.3
-
 (S)-2-methylbutyryl-CoA wild-type enzyme and mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
0.32
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
1.5
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
electron transferring flavoprotein
-
 Rattus norvegicus

General Information

General Information Comment Organism
evolution the enzyme belongs to acyl-CoA dehydrogenase (ACD) family Rattus norvegicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.045
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
0.086
-
 (S)-2-methylbutyryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
0.1
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
0.16
-
 (S)-2-methylbutyryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
0.53
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus