Cloned (Comment) | Organism |
---|---|
- |
Thauera aromatica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-benzylsuccinyl-CoA | complete inhibition | Thauera aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
(R)-2-benzylsuccinyl-CoA | - |
Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
alpha4, 4 * 45000, SDS-PAGE | Thauera aromatica |
200000 | - |
gel filtration | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein | Thauera aromatica | - |
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum tolulyticum | - |
grown on toluene | - |
Aromatoleum tolulyticum B5 | - |
grown on toluene | - |
Azoarcus sp. | - |
strains T and M3, grown on toluene or m-xylene | - |
Thauera aromatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme | Thauera aromatica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.4 | - |
recombinant enzyme | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein | - |
Thauera aromatica | (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein | - |
? | |
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein | highly specific for (R)-benzylsuccinyl-CoA | Thauera aromatica | (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein | - |
? | |
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate | low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA | Thauera aromatica | (E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 45000, SDS-PAGE | Thauera aromatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thauera aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | no FMN or riboflavin detected | Thauera aromatica |