BRENDA - Enzyme Database
show all sequences of 1.3.8.3

(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway

Leutwein, C.; Heider, J.; Arch. Microbiol. 178, 517-524 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Thauera aromatica
Inhibitors
Inhibitors
Commentary
Organism
Structure
(S)-benzylsuccinyl-CoA
complete inhibition
Thauera aromatica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
(R)-2-benzylsuccinyl-CoA
-
Thauera aromatica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
alpha4, 4 * 45000, SDS-PAGE
Thauera aromatica
200000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
Thauera aromatica
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Aromatoleum tolulyticum
-
grown on toluene
-
Aromatoleum tolulyticum B5
-
grown on toluene
-
Azoarcus sp.
-
strains T and M3, grown on toluene or m-xylene
-
Thauera aromatica
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme
Thauera aromatica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4
-
recombinant enzyme
Thauera aromatica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
-
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
highly specific for (R)-benzylsuccinyl-CoA
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
636487
Thauera aromatica
?
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate
low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
-
636487
Thauera aromatica
?
Subunits
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 45000, SDS-PAGE
Thauera aromatica
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thauera aromatica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Thauera aromatica
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
no FMN or riboflavin detected
Thauera aromatica
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thauera aromatica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
no FMN or riboflavin detected
Thauera aromatica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(S)-benzylsuccinyl-CoA
complete inhibition
Thauera aromatica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
(R)-2-benzylsuccinyl-CoA
-
Thauera aromatica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
alpha4, 4 * 45000, SDS-PAGE
Thauera aromatica
200000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
Thauera aromatica
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme
Thauera aromatica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4
-
recombinant enzyme
Thauera aromatica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
-
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
-
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
highly specific for (R)-benzylsuccinyl-CoA
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
636487
Thauera aromatica
?
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate
low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA
636487
Thauera aromatica
(E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
-
636487
Thauera aromatica
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
alpha4, 4 * 45000, SDS-PAGE
Thauera aromatica
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Thauera aromatica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Thauera aromatica
Other publictions for EC 1.3.8.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742539
Chaurasia
Genetic evidence that the deg ...
Geobacter metallireducens, Geobacter metallireducens DSM 7210
FEMS Microbiol. Lett.
362
pii: fnv145
2015
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1
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1
1
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636487
Leutwein
(R)-Benzylsuccinyl-CoA dehydro ...
Azoarcus sp., Aromatoleum tolulyticum, Thauera aromatica, Aromatoleum tolulyticum B5
Arch. Microbiol.
178
517-524
2002
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1
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1
1
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2
1
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8
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1
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1
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3
1
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1
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1
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1
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1
1
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1
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1
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2
1
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1
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1
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3
1
1
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1
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636486
Leuthner
Anaerobic toluene catabolism o ...
Thauera aromatica
J. Bacteriol.
182
272-277
2000
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1
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1
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1
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2
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1
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1
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2
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