Literature summary for 1.3.8.3 extracted from

  • Leutwein, C.; Heider, J.
    (R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway (2002), Arch. Microbiol., 178, 517-524.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thauera aromatica

Inhibitors

Inhibitors Comment Organism Structure
(S)-benzylsuccinyl-CoA complete inhibition Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
(R)-2-benzylsuccinyl-CoA
-
Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Thauera aromatica
45000
-
alpha4, 4 * 45000, SDS-PAGE Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein Thauera aromatica
-
(E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
?

Organism

Organism UniProt Comment Textmining
Azoarcus sp.
-
strains T and M3, grown on toluene or m-xylene
-
Aromatoleum tolulyticum
-
grown on toluene
-
Thauera aromatica
-
-
-
Aromatoleum tolulyticum B5
-
grown on toluene
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, hydroxyapatite, butyl-Fractogel, Superdex HR200, recombinant enzyme Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4
-
recombinant enzyme Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein
-
Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + electron-transferring flavoprotein highly specific for (R)-benzylsuccinyl-CoA Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + reduced electron-transferring flavoprotein
-
?
(R)-2-benzylsuccinyl-CoA + ferricenium hexafluorophosphate low activity with a mixture of phenazine methosulfate/dichlorophenol indophenol, no activity with NAD+, NADP+, dichlorophenolindophenol, ferricyanide or tetrazolium as electron acceptors, very low activity with valeryl-CoA and octanoyl-CoA Thauera aromatica (E)-2-benzylidenesuccinyl-CoA + ferrocenium hexafluorophosphate
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 45000, SDS-PAGE Thauera aromatica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
FAD no FMN or riboflavin detected Thauera aromatica