Literature summary for 1.3.8.12 extracted from

  • Erb, T.J.; Fuchs, G.; Alber, B.E.
    (2S)-Methylsuccinyl-CoA dehydrogenase closes the ethylmalonyl-CoA pathway for acetyl-CoA assimilation (2009), Mol. Microbiol., 73, 992-1008.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a N-terminal histidine-tagged fusion protein Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Cereibacter sphaeroides
62300
-
2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein Cereibacter sphaeroides the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides D3JV03
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell
-
Cereibacter sphaeroides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation Cereibacter sphaeroides 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein the enzyme is highly specific for (S)-methylsuccinyl-CoA. No activity with butyryl-CoA, isobutyryl-CoA or a diastereomeric mixture of (R)-methylsuccinyl-CoA Cereibacter sphaeroides 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
MCD
-
Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains 0.7 mol of noncovalently bound FAD molecule per subunit Cereibacter sphaeroides

General Information

General Information Comment Organism
metabolism the enzyme is involved in the ethylmalonyl-CoA pathway, an acetyl-CoA assimilation strategy Cereibacter sphaeroides