BRENDA - Enzyme Database
show all sequences of 1.3.8.11

Cyclohexanecarboxyl-coenzyme a (CoA) and cyclohex-1-ene-1-carboxyl-CoA dehydrogenases, two enzymes involved in the fermentation of benzoate and crotonate in Syntrophus aciditrophicus

Kung, J.; Seifert, J.; von Bergen, M.; Bolla, M.; J. Bacteriol. 195, 3193-3200 (2013) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells
Syntrophus aciditrophicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cyclohex-1-ene-1-carbonyl-CoA
-
Syntrophus aciditrophicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
cyclohexane-1-carbonyl-CoA
at pH 7.0 and 30°C
Syntrophus aciditrophicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, calculated from amino acid sequence
Syntrophus aciditrophicus
150000
-
recombinant enzyme, gel filtration
Syntrophus aciditrophicus
155000
-
native enzyme, gel filtration
Syntrophus aciditrophicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
Syntrophus aciditrophicus
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
Syntrophus aciditrophicus SB
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Syntrophus aciditrophicus
-
-
-
Syntrophus aciditrophicus SB
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography
Syntrophus aciditrophicus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1
-
cell extract, at pH 7.0 and 30°C
Syntrophus aciditrophicus
52
-
after 25fold purification, at pH 7.0 and 30°C
Syntrophus aciditrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
725285
Syntrophus aciditrophicus
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
725285
Syntrophus aciditrophicus SB
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 42000, calculated from amino acid sequence
Syntrophus aciditrophicus
Synonyms
Synonyms
Commentary
Organism
ChCoA dehydrogenase
-
Syntrophus aciditrophicus
cyclohexanecarboxyl-CoA dehydrogenase
-
Syntrophus aciditrophicus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Syntrophus aciditrophicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.049
-
cyclohex-1-ene-1-carbonyl-CoA
at pH 7.0 and 30°C
Syntrophus aciditrophicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells
Syntrophus aciditrophicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Syntrophus aciditrophicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyclohex-1-ene-1-carbonyl-CoA
-
Syntrophus aciditrophicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.049
-
cyclohex-1-ene-1-carbonyl-CoA
at pH 7.0 and 30°C
Syntrophus aciditrophicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.022
-
cyclohexane-1-carbonyl-CoA
at pH 7.0 and 30°C
Syntrophus aciditrophicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
4 * 42000, calculated from amino acid sequence
Syntrophus aciditrophicus
150000
-
recombinant enzyme, gel filtration
Syntrophus aciditrophicus
155000
-
native enzyme, gel filtration
Syntrophus aciditrophicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
Syntrophus aciditrophicus
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
Syntrophus aciditrophicus SB
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography
Syntrophus aciditrophicus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1
-
cell extract, at pH 7.0 and 30°C
Syntrophus aciditrophicus
52
-
after 25fold purification, at pH 7.0 and 30°C
Syntrophus aciditrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
725285
Syntrophus aciditrophicus
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
725285
Syntrophus aciditrophicus SB
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 42000, calculated from amino acid sequence
Syntrophus aciditrophicus
Other publictions for EC 1.3.8.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742783
Kung
Enzymes involved in a novel a ...
Geobacter metallireducens, Geobacter metallireducens DSM 7210
J. Bacteriol.
196
3667-3674
2014
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
1
-
6
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725285
Kung
Cyclohexanecarboxyl-coenzyme a ...
Syntrophus aciditrophicus, Syntrophus aciditrophicus SB
J. Bacteriol.
195
3193-3200
2013
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-
1
-
-
-
1
1
-
-
3
2
-
5
-
-
1
-
-
-
2
-
2
1
2
-
-
-
-
-
-
-
1
1
-
-
-
-
1
1
-
-
-
-
1
1
1
-
-
3
2
-
-
-
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
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