Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.8.11 extracted from

  • Kung, J.; Seifert, J.; von Bergen, M.; Bolla, M.
    Cyclohexanecarboxyl-coenzyme a (CoA) and cyclohex-1-ene-1-carboxyl-CoA dehydrogenases, two enzymes involved in the fermentation of benzoate and crotonate in Syntrophus aciditrophicus (2013), J. Bacteriol., 195, 3193-3200.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells Syntrophus aciditrophicus

Inhibitors

Inhibitors Comment Organism Structure
cyclohex-1-ene-1-carbonyl-CoA
-
Syntrophus aciditrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
cyclohexane-1-carbonyl-CoA at pH 7.0 and 30°C Syntrophus aciditrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
4 * 42000, calculated from amino acid sequence Syntrophus aciditrophicus
150000
-
recombinant enzyme, gel filtration Syntrophus aciditrophicus
155000
-
native enzyme, gel filtration Syntrophus aciditrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate Syntrophus aciditrophicus
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate Syntrophus aciditrophicus SB
-
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?

Organism

Organism UniProt Comment Textmining
Syntrophus aciditrophicus
-
-
-
Syntrophus aciditrophicus SB
-
-
-

Purification (Commentary)

Purification (Comment) Organism
octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography Syntrophus aciditrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
cell extract, at pH 7.0 and 30°C Syntrophus aciditrophicus
52
-
after 25fold purification, at pH 7.0 and 30°C Syntrophus aciditrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Syntrophus aciditrophicus cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Syntrophus aciditrophicus SB cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 42000, calculated from amino acid sequence Syntrophus aciditrophicus

Synonyms

Synonyms Comment Organism
ChCoA dehydrogenase
-
Syntrophus aciditrophicus
cyclohexanecarboxyl-CoA dehydrogenase
-
Syntrophus aciditrophicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Syntrophus aciditrophicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.049
-
cyclohex-1-ene-1-carbonyl-CoA at pH 7.0 and 30°C Syntrophus aciditrophicus