Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells | Syntrophus aciditrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclohex-1-ene-1-carbonyl-CoA | - |
Syntrophus aciditrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
cyclohexane-1-carbonyl-CoA | at pH 7.0 and 30°C | Syntrophus aciditrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, calculated from amino acid sequence | Syntrophus aciditrophicus |
150000 | - |
recombinant enzyme, gel filtration | Syntrophus aciditrophicus |
155000 | - |
native enzyme, gel filtration | Syntrophus aciditrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate | Syntrophus aciditrophicus | - |
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate | - |
? | |
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate | Syntrophus aciditrophicus SB | - |
cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Syntrophus aciditrophicus | - |
- |
- |
Syntrophus aciditrophicus SB | - |
- |
- |
Purification (Comment) | Organism |
---|---|
octyl Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q ion exchange column chromatography | Syntrophus aciditrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
cell extract, at pH 7.0 and 30°C | Syntrophus aciditrophicus |
52 | - |
after 25fold purification, at pH 7.0 and 30°C | Syntrophus aciditrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate | - |
Syntrophus aciditrophicus | cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate | - |
? | |
cyclohexane-1-carbonyl-CoA + ferricenium hexafluorophosphate | - |
Syntrophus aciditrophicus SB | cyclohex-1-ene-1-carbonyl-CoA + ferrocenium hexafluorophosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 42000, calculated from amino acid sequence | Syntrophus aciditrophicus |
Synonyms | Comment | Organism |
---|---|---|
ChCoA dehydrogenase | - |
Syntrophus aciditrophicus |
cyclohexanecarboxyl-CoA dehydrogenase | - |
Syntrophus aciditrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Syntrophus aciditrophicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
cyclohex-1-ene-1-carbonyl-CoA | at pH 7.0 and 30°C | Syntrophus aciditrophicus |