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Literature summary for 1.3.8.1 extracted from
- Biochemical and electrochemical characterization of two variant human short-chain acyl-CoA dehydrogenases (2005), Biochemistry, 44, 16035-16042.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | common variant SCAD enzymes and their potential contribution to clinical disease in humans | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into Escherichia coli XL1 Blue | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G185S | impairments of both its kinetic and electron transfer properties | Homo sapiens |
| R147W | exhibits almost identical physical and redox properties to wild-type but only half of the specific activity and substrate activation shifts observed in wild-type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
butyryl-CoA | wild-type | Homo sapiens |  |
| 0.065 | - |
butyryl-CoA | R147W mutant | Homo sapiens | |
| 0.124 | - |
butyryl-CoA | G185S mutant | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration, to more than 95% purity | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, phosphate buffer, pH 7.6, 20% glycerol | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyryl-CoA + electron acceptor | - |
Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
| crotonyl-CoA + electron acceptor | - |
Homo sapiens | butyryl-CoA + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SCAD | - |
Homo sapiens |
| short-chain acyl-CoA dehydrogenase | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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