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Literature summary for 1.3.8.1 extracted from

  • Van Berkel, W.J.H.; Van den Berg, W.A.M.; Muller, F.
    Large-scale preparation and reconstitution of apo-flavoproteins with special reference to butyryl-CoA dehydrogenase from Megasphaera elsdenii. Hydrophobic-interaction chromatography [published erratum appears in Eur J Biochem 1989 Apr 1;180(3):603] (1988), Eur. J. Biochem., 178, 197-207.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA 0.5 mM, 60% inhibition, apo-enzyme reconstituted with FAD Megasphaera elsdenii

Organism

Organism UniProt Comment Textmining
Megasphaera elsdenii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
preparation of apoprotein Megasphaera elsdenii

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of holoenzyme after removal of FAD Megasphaera elsdenii

Storage Stability

Storage Stability Organism
-18°C, 50% ethylene glycol, 6 months, 80% activity after reconstitution with FAD Megasphaera elsdenii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
most stable Megasphaera elsdenii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00012
-
acetoacetyl-CoA apo-enzyme reconstituted with FAD Megasphaera elsdenii