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Literature summary for 1.3.7.8 extracted from

  • Anselmann, S.; Loeffler, C.; Staerk, H.; Jehmlich, N.; von Bergen, M.; Bruels, T.; Boll, M.
    The class II benzoyl-coenzyme A reductase complex from the sulfate-reducing Desulfosarcina cetonica (2019), Environ. Microbiol., 21, 4241-4252 .
    View publication on PubMed
Search for more literature for 1.3.7.8

Cloned(Commentary)

Cloned (Comment) Organism
identification of multiple copies of putative genes encoding the structural components of a class II BCR in sulfate reducing, Fe(III)-respiring and syntrophic bacteria Desulfosarcina cetonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme shows Michaelis-Menten kinetics Desulfosarcina cetonica

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 185 per enzyme complex Desulfosarcina cetonica
additional information theoretical analyses show 4 W-pterins, selenocysteines, 6 flavin adenine dinucleotides, 4 Zn, and 48 Fe-S clusters per enzyme complex Desulfosarcina cetonica
Tungsten 4 W per enzyme complex, in the active site pterin cofactor Desulfosarcina cetonica
Zn2+ 3.4 per enzyme complex Desulfosarcina cetonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
950000
-
 soluble Bam[(BC)2DEFGHI]2 complex Desulfosarcina cetonica
956000
-
 amino acid sequence calculation Desulfosarcina cetonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Desulfosarcina cetonica high activity cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Desulfosarcina cetonica DSMZ 7267 high activity cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O Desulfosarcina cetonica 480 high activity cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Desulfosarcina cetonica
-
-
-
Desulfosarcina cetonica 480
-
-
-
Desulfosarcina cetonica DSMZ 7267
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from Desulfosarcina cetonica cells grown on benzoate and sulfate 17.3fold by anion exchange chromatography and gel filtration Desulfosarcina cetonica

Reaction

Reaction Comment Organism Reaction ID
cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate = benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O class II BCRs drive BzCoA reduction by flavin-based electron bifurcation (FBEB) Desulfosarcina cetonica
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity (reverse reaction), purified native enzyme, pH 6.0, temperature not specified in the publication Desulfosarcina cetonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O high activity Desulfosarcina cetonica cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O high activity Desulfosarcina cetonica DSMZ 7267 cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
benzoyl-CoA + reduced ferredoxin + 2 ATP + 2 H2O high activity Desulfosarcina cetonica 480 cyclohexa-1,5-diene-1-carbonyl-CoA + oxidized ferredoxin + 2 ADP + 2 phosphate
-
 ?
additional information class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation Desulfosarcina cetonica ?
-
-
additional information class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation Desulfosarcina cetonica DSMZ 7267 ?
-
-
additional information class II BCR from Desulfosarcina cetonica slowly disproportionates two 1,5-dienoyl-CoA to BzCoA and 1-monenoyl-CoA at about 0.1% of the 1,5-dienoyl-CoA:methyl viologen oxidoreductase (DCO) activity rate. Assays for BzCoA reduction are perfomred by flavin-based electron bifurcation Desulfosarcina cetonica 480 ?
-
-

Subunits

Subunits Comment Organism
More the class II BCR complex is composed of the BamBCDEGHI subunits Desulfosarcina cetonica

Synonyms

Synonyms Comment Organism
1,5-dienoyl-CoA:methyl viologen oxidoreductase
-
 Desulfosarcina cetonica
BCR
-
 Desulfosarcina cetonica
class II BCR complex
-
 Desulfosarcina cetonica
class II benzoyl-coenzyme A reductase complex
-
 Desulfosarcina cetonica
DCO
-
 Desulfosarcina cetonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Desulfosarcina cetonica

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Desulfosarcina cetonica
FAD 4.8 FAD per enzyme complex Desulfosarcina cetonica
Fe-S center 48 Fe-S clusters per enzyme complex. The BamC subunit harbours three electron-transferring [4Fe-4S] clusters Desulfosarcina cetonica
tungsto-bispterin cofactor
-
 Desulfosarcina cetonica

General Information

General Information Comment Organism
evolution high-molecular class II BCR metalloenzyme machineries are remarkably conserved in strictly anaerobic bacteria with regard to subunit architecture and cofactor content, but their subcellular localization and electron acceptor preference may differ as a result of adaptations to variable energy metabolisms. There are two non-related classes of BCRs that follow fundamentally different strategies for BzCoA dearomatization. Class I BCRs couple electron transfer from a reduced ferredoxin or the aromatic ring to a stoichiometric hydrolysis of two MgATP. These homotetrameric, three [4Fe-4S] cluster containing enzymes are composed of an ATP-hydrolyzing module composed of two highly similar subunits and a heterodimeric BzCoA reducing module. Class I BCRs are abundant in facultatively anaerobic bacteria and have been isolated and characterized from aromatic compound degrading, denitrifying Thauera species. The ATP-independent class II BCRs occur in strictly anaerobic sulfate-, metal-oxide-reducing or syntrophic bacteria that gain far less energy during the oxidation of aromatics to CO2 or acetate. Class II BCR occur in the Fe(III)-respiring Geobacter metallireducens Desulfosarcina cetonica
metabolism most monocyclic aromatic compounds including the BTEX (benzene, toluene, ethylbenzene and xylenes) are first converted in channelling enzymatic reaction sequences to the central intermediate benzoyl-coenzyme A (BzCoA), which then serves as substrate for dearomatizing cyclohexa-1,5-diene-1-carboxyl-CoA (1,5-dienoyl-CoA) forming BzCoA reductases (BCRs) Desulfosarcina cetonica
physiological function benzoyl-CoA reductases (BCRs) catalyse a key reaction in the anaerobic degradation pathways of monocyclic aromatic substrates, the dearomatization of benzoyl-CoA (BzCoA) to cyclohexa-1,5-diene-1-carboxyl-CoA (1,5-dienoyl-CoA) at the negative redox potential limit of diffusible enzymatic substrate/product couples. The class II BCR complex composed of BamBCDEGHI subunits is supposed to drive endergonic benzene ring reduction at an active site W-pterin cofactor by flavin-based electron bifurcation Desulfosarcina cetonica