BRENDA - Enzyme Database show
show all sequences of 1.3.7.8

Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thaurea aromatica strain K172

Boll, M.; Fuchs, G.; Eur. J. Biochem. 234, 921-933 (1995)

Data extracted from this reference:

General Stability
General Stability
Organism
oxygen-sensitive
Thauera aromatica
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridyl
-
Thauera aromatica
Acetylene
-
Thauera aromatica
ATP
above 12 mM
Thauera aromatica
ethylene
-
Thauera aromatica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
benzoyl-CoA
-
Thauera aromatica
0.15
-
NH2OH
-
Thauera aromatica
0.6
-
ATP
-
Thauera aromatica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
15% of the activity with Mg2+
Thauera aromatica
Fe2+
35% of the activity with Mg2+
Thauera aromatica
Iron
contains 10.8 mol Fe and 10.5 mol acid-labile sulfur per mol of enzyme
Thauera aromatica
Mg2+
specific for MgATP2-
Thauera aromatica
Mn2+
52% of the activity with Mg2+
Thauera aromatica
Zinc
contains 0.5 mol of Zn per mol of enzyme
Thauera aromatica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
38000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
45000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
48000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
170000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Benzoyl-CoA + reduced acceptor + ATP
Thauera aromatica
key enzyme of anaerobic aromatic metabolism
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera aromatica
-
-
-
Purification (Commentary)
Commentary
Organism
-
Thauera aromatica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.55
-
-
Thauera aromatica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminobenzoyl-CoA + reduced methyl viologen + ATP
at 18% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 78% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 10% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 23% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
at 12% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-methylbenzoyl-CoA + reduced methyl viologen + ATP
at 12% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
at 5% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate
-
-
-
-
azide + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
benzoyl-CoA + reduced acceptor + ATP
acceptor: Ti3+, reduced methyl viologen
12559
Thauera aromatica
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
12559
Thauera aromatica
-
Benzoyl-CoA + reduced acceptor + ATP
key enzyme of anaerobic aromatic metabolism
12559
Thauera aromatica
?
-
-
-
-
additional information
in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity
12559
Thauera aromatica
?
-
-
-
-
N-methylhydroxylamine + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
NH2OH + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
O-methylhydroxylamine + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
7.5
-
Thauera aromatica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.6
8.2
50% of maximal activity at pH 6.6 and 8.2, less than 10% of maximal activity at pH 6.2 and 8.7
Thauera aromatica
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
contains 0.3 mol flavin or flavin-like compound per mol of enzyme
Thauera aromatica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
contains 0.3 mol flavin or flavin-like compound per mol of enzyme
Thauera aromatica
General Stability (protein specific)
General Stability
Organism
oxygen-sensitive
Thauera aromatica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-bipyridyl
-
Thauera aromatica
Acetylene
-
Thauera aromatica
ATP
above 12 mM
Thauera aromatica
ethylene
-
Thauera aromatica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
benzoyl-CoA
-
Thauera aromatica
0.15
-
NH2OH
-
Thauera aromatica
0.6
-
ATP
-
Thauera aromatica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
15% of the activity with Mg2+
Thauera aromatica
Fe2+
35% of the activity with Mg2+
Thauera aromatica
Iron
contains 10.8 mol Fe and 10.5 mol acid-labile sulfur per mol of enzyme
Thauera aromatica
Mg2+
specific for MgATP2-
Thauera aromatica
Mn2+
52% of the activity with Mg2+
Thauera aromatica
Zinc
contains 0.5 mol of Zn per mol of enzyme
Thauera aromatica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
38000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
45000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
48000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
170000
-
gel filtration
Thauera aromatica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
Benzoyl-CoA + reduced acceptor + ATP
Thauera aromatica
key enzyme of anaerobic aromatic metabolism
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thauera aromatica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.55
-
-
Thauera aromatica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminobenzoyl-CoA + reduced methyl viologen + ATP
at 18% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 78% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 10% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP
at 23% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
at 12% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
3-methylbenzoyl-CoA + reduced methyl viologen + ATP
at 12% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
?
-
-
-
-
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP
at 5% of the activity relative to benzoyl-CoA
12559
Thauera aromatica
4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate
-
-
-
-
azide + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
benzoyl-CoA + reduced acceptor + ATP
acceptor: Ti3+, reduced methyl viologen
12559
Thauera aromatica
cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
12559
Thauera aromatica
-
Benzoyl-CoA + reduced acceptor + ATP
key enzyme of anaerobic aromatic metabolism
12559
Thauera aromatica
?
-
-
-
-
additional information
in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity
12559
Thauera aromatica
?
-
-
-
-
N-methylhydroxylamine + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
NH2OH + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
O-methylhydroxylamine + ATP + H2O
-
12559
Thauera aromatica
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE
Thauera aromatica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
7.5
-
Thauera aromatica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.6
8.2
50% of maximal activity at pH 6.6 and 8.2, less than 10% of maximal activity at pH 6.2 and 8.7
Thauera aromatica
Other publictions for EC 1.3.7.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729651
Schmid
Enzymes of the benzoyl-coenzym ...
Ferroglobus placidus
Environ. Microbiol.
17
3289-3300
2015
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5
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1
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726004
Kuntze
Enzymes involved in the anaero ...
Thauera chlorobenzoica, Thauera chlorobenzoica 3CB-1
Mol. Microbiol.
82
758-769
2011
1
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1
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1
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9
-
3
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6
-
12
-
1
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1
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2
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1
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2
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1
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1
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9
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6
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12
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1
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1
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1
2
2
1
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700199
Heintz
Differential membrane proteome ...
Geobacter metallireducens
Mol. Cell. Proteomics
8
2159-2169
2009
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1
4
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1
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4
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1
4
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1
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1
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692753
Thiele
Mechanism of enzymatic Birch r ...
Thauera aromatica
J. Am. Chem. Soc.
130
14050-14051
2008
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1
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2
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1
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1
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1
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1
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1
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674313
Peres
BadM is a transcriptional repr ...
Rhodopseudomonas palustris
J. Bacteriol.
188
8662-8665
2006
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1
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2
1
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1
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1
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671412
Song
Genetic diversity of benzoyl c ...
Acidovorax sp., Acidovorax sp. 2FB7, Azoarcus evansii, Azoarcus toluclasticus, Azoarcus tolulyticus, Azoarcus toluvorans, Magnetospirillum magnetotacticum, Rhodopseudomonas palustris, Thauera aromatica, Thauera chlorobenzoica, Thauera chlorobenzoica 3CB-1, Thauera selenatis, Thauera selenatis 3CB-1
Appl. Environ. Microbiol.
71
2036-2045
2005
-
10
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20
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30
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20
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17
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20
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20
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676054
Carmona
Iron-reducing bacteria unravel ...
Azoarcus sp., Thauera aromatica
Mol. Microbiol.
58
1210-1215
2005
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2
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4
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654733
Mobitz
Substrate binding and reductio ...
Thauera aromatica
Biochemistry
43
1376-1385
2004
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1
11
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3
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1
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12
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1
1
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1
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1
1
11
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12
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1
1
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657182
Unciuleac
Mechanism of ATP-driven electr ...
Thauera aromatica
Proc. Natl. Acad. Sci. USA
98
13619-13624
2001
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285200
Boll
Nonaromatic products from anox ...
Thauera aromatica
J. Biol. Chem.
275
21889-21895
2000
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12557
Boll
Identification, and characteri ...
Thauera aromatica
Eur. J. Biochem.
251
946-954
1998
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1
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1
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1
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1
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12562
Breese
Genes coding the benzoyl-CoA p ...
Thauera aromatica
Eur. J. Biochem.
256
148-154
1998
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288500
Heider
Differential induction of enzy ...
Thauera aromatica
Arch. Microbiol.
170
120-131
1998
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718
Schneider
Anaerobic metabolism of L-phen ...
Thauera aromatica
Arch. Microbiol.
168
310-320
1997
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4
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1
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1
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1
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1
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12558
Boll
Benzoyl-CoA reductase (dearoma ...
Thauera aromatica, Thauera aromatica K172
Eur. J. Biochem.
244
840-851
1997
2
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1
2
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2
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2
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5
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1
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6
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2
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1
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2
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2
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2
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1
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6
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12559
Boll
Benzoyl-coenzyme A reductase ( ...
Thauera aromatica
Eur. J. Biochem.
234
921-933
1995
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1
4
3
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6
5
1
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5
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1
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1
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14
1
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1
1
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1
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1
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1
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4
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3
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6
5
1
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1
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1
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14
1
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1
1
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12563
Koch
Enzymatic reduction of benzoyl ...
Pseudomonas sp.
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195-202
1992
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1
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1
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3
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1
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1
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1
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1
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1
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1
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