General Stability | Organism |
---|---|
oxygen-sensitive | Thauera aromatica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | - |
Thauera aromatica | |
Acetylene | - |
Thauera aromatica | |
ATP | above 12 mM | Thauera aromatica | |
ethylene | - |
Thauera aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
benzoyl-CoA | - |
Thauera aromatica | |
0.15 | - |
NH2OH | - |
Thauera aromatica | |
0.6 | - |
ATP | - |
Thauera aromatica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 15% of the activity with Mg2+ | Thauera aromatica | |
Fe2+ | 35% of the activity with Mg2+ | Thauera aromatica | |
Iron | contains 10.8 mol Fe and 10.5 mol acid-labile sulfur per mol of enzyme | Thauera aromatica | |
Mg2+ | specific for MgATP2- | Thauera aromatica | |
Mn2+ | 52% of the activity with Mg2+ | Thauera aromatica | |
Zinc | contains 0.5 mol of Zn per mol of enzyme | Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE | Thauera aromatica |
38000 | - |
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE | Thauera aromatica |
45000 | - |
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE | Thauera aromatica |
48000 | - |
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE | Thauera aromatica |
170000 | - |
gel filtration | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-CoA + reduced acceptor + ATP | Thauera aromatica | key enzyme of anaerobic aromatic metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thauera aromatica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
- |
Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-CoA + reduced methyl viologen + ATP | at 18% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP | at 78% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP | at 10% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP | at 23% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP | at 12% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
3-methylbenzoyl-CoA + reduced methyl viologen + ATP | at 12% of the activity relative to benzoyl-CoA | Thauera aromatica | ? | - |
? | |
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP | at 5% of the activity relative to benzoyl-CoA | Thauera aromatica | 4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate | - |
? | |
azide + ATP + H2O | - |
Thauera aromatica | ? | - |
? | |
benzoyl-CoA + reduced acceptor + ATP | acceptor: Ti3+, reduced methyl viologen | Thauera aromatica | cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate | - |
? | |
Benzoyl-CoA + reduced acceptor + ATP | key enzyme of anaerobic aromatic metabolism | Thauera aromatica | ? | - |
? | |
additional information | in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity | Thauera aromatica | ? | - |
? | |
N-methylhydroxylamine + ATP + H2O | - |
Thauera aromatica | ? | - |
? | |
NH2OH + ATP + H2O | - |
Thauera aromatica | ? | - |
? | |
O-methylhydroxylamine + ATP + H2O | - |
Thauera aromatica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE | Thauera aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.5 | - |
Thauera aromatica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.2 | 50% of maximal activity at pH 6.6 and 8.2, less than 10% of maximal activity at pH 6.2 and 8.7 | Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | contains 0.3 mol flavin or flavin-like compound per mol of enzyme | Thauera aromatica |