BRENDA - Enzyme Database show
show all sequences of 1.3.7.7

Crystal structure of the L protein of Rhodobacter sphaeroides light-independent protochlorophyllide reductase with MgADP bound: a homologue of the nitrogenase Fe protein

Sarma, R.; Barney, B.M.; Hamilton, T.L.; Jones, A.; Seefeldt, L.C.; Peters, J.W.; Biochemistry 47, 13004-13015 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Azotobacter vinelandii strain DJ884
Rhodobacter sphaeroides
Crystallization (Commentary)
Crystallization
Organism
L-protein of DPOR with Mg-ADP bound, microcapillary batch diffusion method, with 20-25% (w/v) PEG 3350 as the precipitating agent, with 200 mM magnesium formate (pH 7.7)
Rhodobacter sphaeroides
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
the purified, recombinant protein L contains 3.6 mol of Fe/mol of protein L homodimer, consistent with the presence of a [4Fe-4S] cluster
Rhodobacter sphaeroides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33310
-
calculated from amino acid sequence
Rhodobacter sphaeroides
33380
-
mass spectroscopy
Rhodobacter sphaeroides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
Q9RFD6
-
-
Purification (Commentary)
Commentary
Organism
zinc-loaded chelating Sepharose column chromatography and Q-Sepharose column chromatography
Rhodobacter sphaeroides
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
711198
Rhodobacter sphaeroides
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Rhodobacter sphaeroides
ADP
-
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Azotobacter vinelandii strain DJ884
Rhodobacter sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
4Fe-4S-center
-
Rhodobacter sphaeroides
ADP
-
Rhodobacter sphaeroides
Crystallization (Commentary) (protein specific)
Crystallization
Organism
L-protein of DPOR with Mg-ADP bound, microcapillary batch diffusion method, with 20-25% (w/v) PEG 3350 as the precipitating agent, with 200 mM magnesium formate (pH 7.7)
Rhodobacter sphaeroides
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
the purified, recombinant protein L contains 3.6 mol of Fe/mol of protein L homodimer, consistent with the presence of a [4Fe-4S] cluster
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33310
-
calculated from amino acid sequence
Rhodobacter sphaeroides
33380
-
mass spectroscopy
Rhodobacter sphaeroides
Purification (Commentary) (protein specific)
Commentary
Organism
zinc-loaded chelating Sepharose column chromatography and Q-Sepharose column chromatography
Rhodobacter sphaeroides
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
711198
Rhodobacter sphaeroides
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?
Other publictions for EC 1.3.7.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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743401
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1
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1
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1
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1
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1
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2
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1
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1
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1
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1
-
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-
-
-
-
-
2
2
-
-
-
726394
Moser
Structure of ADP-aluminium flu ...
Prochlorococcus marinus
Proc. Natl. Acad. Sci. USA
110
2094-2098
2013
-
-
-
1
-
-
-
-
-
2
-
1
-
6
-
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1
1
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2
1
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2
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2
1
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2
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1
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1
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2
1
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3
3
-
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-
725926
Nazir
Chloroplast-encoded chlB gene ...
Pinus thunbergii
Mol. Biol. Rep.
39
10637-10646
2012
-
-
1
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
-
-
-
712026
Kondo
EPR study of 1Asp-3Cys ligated ...
Rhodobacter capsulatus
FEBS Lett.
585
214-218
2011
-
-
-
-
1
-
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-
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1
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2
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2
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2
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1
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2
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725877
Moser
Methods for nitrogenase-like d ...
Prochlorococcus marinus
Methods Mol. Biol.
766
129-143
2011
-
-
-
-
-
-
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-
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1
-
1
-
1
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1
1
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1
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1
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1
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1
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1
1
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-
-
-
-
2
2
-
-
-
712465
Broecker
Crystal structure of the nitro ...
Thermosynechococcus elongatus
J. Biol. Chem.
285
27336-27345
2010
-
-
1
1
2
-
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-
2
-
1
-
1
-
-
1
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-
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3
2
-
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-
-
2
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1
2
1
2
-
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2
-
1
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1
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3
2
-
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-
713102
Muraki
X-ray crystal structure of the ...
Rhodobacter capsulatus, Rhodobacter capsulatus DB176
Nature
465
110-114
2010
-
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1
1
7
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1
-
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2
-
2
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3
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1
1
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2
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10
2
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3
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1
3
1
7
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1
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2
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2
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1
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2
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10
2
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1
1
-
-
-
725412
Broecker
Biosynthesis of (bacterio)chlo ...
Prochlorococcus marinus
J. Biol. Chem.
285
8268-8277
2010
-
-
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1
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1
6
2
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1
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3
1
1
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2
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2
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1
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1
6
2
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3
1
1
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1
1
-
-
-
726104
Breznenova¡
-
Light-independent accumulation ...
Pinus mugo, Pinus sylvestris
Photosynthetica
48
16-22
2010
-
-
2
-
-
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-
-
2
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2
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2
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4
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2
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4
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2
4
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2
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2
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4
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2
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4
4
-
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-
726526
Reinbothe
Chlorophyll biosynthesis: spot ...
Chlorobaculum tepidum, Chloroflexus aurantiacus, Heliobacillus mobilis, Prochlorococcus marinus, Rhodobacter capsulatus, Rhodobacter sphaeroides
Trends Plant Sci.
15
614-624
2010
-
-
-
2
3
-
-
-
-
12
-
6
-
12
-
-
-
6
-
-
-
-
6
6
-
-
-
-
-
-
-
12
-
-
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-
12
2
3
-
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12
-
6
-
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6
6
-
-
-
-
-
-
-
-
-
14
14
-
-
-
700718
Shui
Light-dependent and light-inde ...
Microchaete diplosiphon
Plant Cell Physiol.
50
1507-1521
2009
-
-
-
-
-
-
-
-
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7
-
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3
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1
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3
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3
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2
-
-
6
-
-
686758
Nomata
NB-protein (BchN-BchB) of dark ...
Rhodobacter capsulatus
FEBS Lett.
582
1346-1350
2008
-
-
-
-
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1
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2
1
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1
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1
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1
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711198
Sarma
Crystal structure of the L pro ...
Rhodobacter sphaeroides
Biochemistry
47
13004-13015
2008
-
-
1
1
-
-
-
-
-
1
2
-
-
3
-
-
1
-
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1
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2
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1
2
1
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1
2
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1
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1
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672764
Shi
Characterization of three gene ...
Auxenochlorella protothecoides
Biotechnol. Prog.
22
1050-1055
2006
-
-
1
-
-
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1
-
3
1
-
8
-
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1
1
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2
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1
2
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1
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3
1
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1
1
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1
1
1
1
-
-
712984
Kusumi
Proceedings of the SMBE Tri-Na ...
Chamaecyparis lawsoniana, Chamaecyparis obtusa, Chamaecyparis pisifera, Cryptomeria japonica, Cunninghamia lanceolata, Cupressus sempervirens, Glyptostrobus pensilis, Juniperus chinensis, Juniperus rigida, Metasequoia glyptostroboides, no activity in Thuja occidentalis, no activity in Thuja plicata, no activity in Thuja standishii, Platycladus orientalis, Sequoiadendron giganteum, Sequoia sempervirens, Taxodium distichum, Thujopsis dolabrata
Mol. Biol. Evol.
23
941-948
2006
-
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20
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15
15
-
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-
672324
Nomata
Overexpression and characteriz ...
Rhodobacter capsulatus
Biochim. Biophys. Acta
1708
229-237
2005
-
-
1
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1
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8
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2
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1
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2
1
2
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1
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1
1
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1
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8
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1
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2
1
2
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713305
Raskin
Experimental approach to eluci ...
no activity in Hordeum vulgare
Plant Physiol.
133
25-28
2003
-
-
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-
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1
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393862
Fujita
Reconstitution of light-indepe ...
Rhodobacter capsulatus
J. Biol. Chem.
275
23583-23588
2000
1
-
1
-
-
-
-
-
-
-
6
-
-
3
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
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-
-
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-
713293
Skinner
Differential expression of gen ...
Pinus taeda
Plant Mol. Biol.
39
577-592
1999
-
-
-
-
-
-
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-
1
-
-
-
-
3
-
-
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3
-
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-
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1
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-
3
-
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-
-
-
-
-
-
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-
-
1
-
-
1
-
-
713252
Fujita
Cloning of the gene encoding a ...
Leptolyngbya boryana
Plant Cell Physiol.
39
177-185
1998
-
-
-
-
-
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1
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1
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1
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1
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1
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bchFNBH bacteriochlorophyll sy ...
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