BRENDA - Enzyme Database show
show all sequences of 1.3.7.7

Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus

Nomata, J.; Swem, L.R.; Bauer, C.E.; Fujita, Y.; Biochim. Biophys. Acta 1708, 229-237 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Rhodobacter capsulatus mutant DB176 cells
Rhodobacter capsulatus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0106
-
protochlorophyllide
in 100 mM HEPES-KOH (pH 7.4), 5 mM MgCl2, 5 mM dithiothreitol, at 34°C
Rhodobacter capsulatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, S-tag subunit BchL, SDS-PAGE
Rhodobacter capsulatus
36046
-
2 * 36046, subunit BchL calculated from amino acid sequence
Rhodobacter capsulatus
48671
-
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence
Rhodobacter capsulatus
52000
-
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
57191
-
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence
Rhodobacter capsulatus
60000
-
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
67000
-
S-tag subunit BchL, gel filtration
Rhodobacter capsulatus
200000
-
NB-protein complex, gel filtration
Rhodobacter capsulatus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter capsulatus
-
-
-
Purification (Commentary)
Commentary
Organism
S-protein agarose column chromatography
Rhodobacter capsulatus
Storage Stability
Storage Stability
Organism
4°C, NB- and L-protein subunits of the crude extract when maintained anaerobically, more than 6 months, remain stable with no significant loss of activity
Rhodobacter capsulatus
4°C, NB- and L-protein subunits of the purified extract when maintained anaerobically, both activities of the components are rapidly lost during purification procedures such as affinity chromatography with S-protein agarose
Rhodobacter capsulatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
672324
Rhodobacter capsulatus
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterotetramer
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence; 2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
homodimer
2 * 36000, S-tag subunit BchL, SDS-PAGE; 2 * 36046, subunit BchL calculated from amino acid sequence
Rhodobacter capsulatus
Cofactor
Cofactor
Commentary
Organism
Structure
ADP
-
Rhodobacter capsulatus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Rhodobacter capsulatus mutant DB176 cells
Rhodobacter capsulatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ADP
-
Rhodobacter capsulatus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0106
-
protochlorophyllide
in 100 mM HEPES-KOH (pH 7.4), 5 mM MgCl2, 5 mM dithiothreitol, at 34°C
Rhodobacter capsulatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
2 * 36000, S-tag subunit BchL, SDS-PAGE
Rhodobacter capsulatus
36046
-
2 * 36046, subunit BchL calculated from amino acid sequence
Rhodobacter capsulatus
48671
-
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence
Rhodobacter capsulatus
52000
-
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
57191
-
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence
Rhodobacter capsulatus
60000
-
2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
67000
-
S-tag subunit BchL, gel filtration
Rhodobacter capsulatus
200000
-
NB-protein complex, gel filtration
Rhodobacter capsulatus
Purification (Commentary) (protein specific)
Commentary
Organism
S-protein agarose column chromatography
Rhodobacter capsulatus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, NB- and L-protein subunits of the crude extract when maintained anaerobically, more than 6 months, remain stable with no significant loss of activity
Rhodobacter capsulatus
4°C, NB- and L-protein subunits of the purified extract when maintained anaerobically, both activities of the components are rapidly lost during purification procedures such as affinity chromatography with S-protein agarose
Rhodobacter capsulatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
672324
Rhodobacter capsulatus
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterotetramer
2 * 48671 + 2 * 57191, subunit BchN and subunit BchB, calculated from amino acid sequence; 2 * 52000 + 2 * 60000, NB-protein complex, SDS-PAGE
Rhodobacter capsulatus
homodimer
2 * 36000, S-tag subunit BchL, SDS-PAGE; 2 * 36046, subunit BchL calculated from amino acid sequence
Rhodobacter capsulatus
Other publictions for EC 1.3.7.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743315
Zhang
The SWI2/SNF2 chromatin-remod ...
Arabidopsis thaliana
Mol. Plant
10
155-167
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
741827
Nomata
Stoichiometry of ATP hydrolys ...
Rhodobacter capsulatus, Rhodobacter capsulatus ATCC BAA-309
Biochem. Biophys. Res. Commun.
470
704-709
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743401
Silva
With or without light compari ...
Rhodobacter capsulatus, Rhodobacter capsulatus ATCC BAA-309
PeerJ
2
e551
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726255
Kopecna
Inhibition of chlorophyll bios ...
Synechocystis sp.
Planta
237
497-508
2013
-
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726394
Moser
Structure of ADP-aluminium flu ...
Prochlorococcus marinus
Proc. Natl. Acad. Sci. USA
110
2094-2098
2013
-
-
-
1
-
-
-
-
-
2
-
1
-
6
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
725926
Nazir
Chloroplast-encoded chlB gene ...
Pinus thunbergii
Mol. Biol. Rep.
39
10637-10646
2012
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712026
Kondo
EPR study of 1Asp-3Cys ligated ...
Rhodobacter capsulatus
FEBS Lett.
585
214-218
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725877
Moser
Methods for nitrogenase-like d ...
Prochlorococcus marinus
Methods Mol. Biol.
766
129-143
2011
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
712465
Broecker
Crystal structure of the nitro ...
Thermosynechococcus elongatus
J. Biol. Chem.
285
27336-27345
2010
-
-
1
1
2
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
-
-
3
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
2
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713102
Muraki
X-ray crystal structure of the ...
Rhodobacter capsulatus, Rhodobacter capsulatus DB176
Nature
465
110-114
2010
-
-
1
1
7
-
1
-
-
2
-
2
-
3
-
-
1
1
-
-
2
-
10
2
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
1
7
-
-
1
-
-
-
2
-
2
-
-
-
1
-
-
2
-
10
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725412
Broecker
Biosynthesis of (bacterio)chlo ...
Prochlorococcus marinus
J. Biol. Chem.
285
8268-8277
2010
-
-
-
-
1
-
-
-
-
1
6
2
-
1
-
-
-
-
-
-
-
-
3
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
6
2
-
-
-
-
-
-
-
-
3
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
726104
Breznenova¡
-
Light-independent accumulation ...
Pinus mugo, Pinus sylvestris
Photosynthetica
48
16-22
2010
-
-
2
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
726526
Reinbothe
Chlorophyll biosynthesis: spot ...
Chlorobaculum tepidum, Chloroflexus aurantiacus, Heliobacillus mobilis, Prochlorococcus marinus, Rhodobacter capsulatus, Rhodobacter sphaeroides
Trends Plant Sci.
15
614-624
2010
-
-
-
2
3
-
-
-
-
12
-
6
-
12
-
-
-
6
-
-
-
-
6
6
-
-
-
-
-
-
-
12
-
-
-
-
-
-
12
2
3
-
-
-
-
-
-
12
-
6
-
-
-
-
-
-
-
-
6
6
-
-
-
-
-
-
-
-
-
14
14
-
-
-
700718
Shui
Light-dependent and light-inde ...
Microchaete diplosiphon
Plant Cell Physiol.
50
1507-1521
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
686758
Nomata
NB-protein (BchN-BchB) of dark ...
Rhodobacter capsulatus
FEBS Lett.
582
1346-1350
2008
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711198
Sarma
Crystal structure of the L pro ...
Rhodobacter sphaeroides
Biochemistry
47
13004-13015
2008
-
-
1
1
-
-
-
-
-
1
2
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672764
Shi
Characterization of three gene ...
Auxenochlorella protothecoides
Biotechnol. Prog.
22
1050-1055
2006
-
-
1
-
-
-
-
-
1
-
3
1
-
8
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
3
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
712984
Kusumi
Proceedings of the SMBE Tri-Na ...
Chamaecyparis lawsoniana, Chamaecyparis obtusa, Chamaecyparis pisifera, Cryptomeria japonica, Cunninghamia lanceolata, Cupressus sempervirens, Glyptostrobus pensilis, Juniperus chinensis, Juniperus rigida, Metasequoia glyptostroboides, no activity in Thuja occidentalis, no activity in Thuja plicata, no activity in Thuja standishii, Platycladus orientalis, Sequoiadendron giganteum, Sequoia sempervirens, Taxodium distichum, Thujopsis dolabrata
Mol. Biol. Evol.
23
941-948
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
15
-
-
-
672324
Nomata
Overexpression and characteriz ...
Rhodobacter capsulatus
Biochim. Biophys. Acta
1708
229-237
2005
-
-
1
-
-
-
-
1
-
-
8
-
-
2
-
-
1
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
8
-
-
-
-
1
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713305
Raskin
Experimental approach to eluci ...
no activity in Hordeum vulgare
Plant Physiol.
133
25-28
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393862
Fujita
Reconstitution of light-indepe ...
Rhodobacter capsulatus
J. Biol. Chem.
275
23583-23588
2000
1
-
1
-
-
-
-
-
-
-
6
-
-
3
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713293
Skinner
Differential expression of gen ...
Pinus taeda
Plant Mol. Biol.
39
577-592
1999
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713252
Fujita
Cloning of the gene encoding a ...
Leptolyngbya boryana
Plant Cell Physiol.
39
177-185
1998
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713251
Fujita
Identification of the chlB gen ...
Leptolyngbya boryana
Plant Cell Physiol.
37
313-323
1996
-
-
1
-
-
-
-
-
1
-
2
1
-
5
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712268
Burke
bchFNBH bacteriochlorophyll sy ...
Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
J. Bacteriol.
175
2414-2422
1993
-
-
-
-
-
-
-
-
-
-
-
4
-
10
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-