BRENDA - Enzyme Database
show all sequences of 1.3.7.6

Attachment of noncognate chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by heterologous expression in Escherichia coli

Alvey, R.M.; Biswas, A.; Schluchter, W.M.; Bryant, D.A.; Biochemistry 50, 4890-4902 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene pebS, production of His-tagged phycocyanin alpha-subunit-(3Z)-phycoerythrobilin using the alternative FDBR, phycoerythrobilin synthase, PebS, overview. Recombinant functional expression of PebS in Escherichia coli, co-expression with phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, cyanobacterial heme oxygenase, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview
Prochlorococcus phage P-SSM4
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
biliverdin IXalpha + 2 reduced ferredoxin
Prochlorococcus phage P-SSM4
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Prochlorococcus phage P-SSM4
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
biliverdin IXalpha + 2 reduced ferredoxin
-
724317
Prochlorococcus phage P-SSM4
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
PebS
-
Prochlorococcus phage P-SSM4
phycoerythrobilin synthase
-
Prochlorococcus phage P-SSM4
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Prochlorococcus phage P-SSM4
Cloned(Commentary) (protein specific)
Commentary
Organism
gene pebS, production of His-tagged phycocyanin alpha-subunit-(3Z)-phycoerythrobilin using the alternative FDBR, phycoerythrobilin synthase, PebS, overview. Recombinant functional expression of PebS in Escherichia coli, co-expression with phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, cyanobacterial heme oxygenase, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview
Prochlorococcus phage P-SSM4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
-
Prochlorococcus phage P-SSM4
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
biliverdin IXalpha + 2 reduced ferredoxin
Prochlorococcus phage P-SSM4
-
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
biliverdin IXalpha + 2 reduced ferredoxin
-
724317
Prochlorococcus phage P-SSM4
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin
-
-
-
?
Other publictions for EC 1.3.7.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724220
Busch
Radical mechanism of cyanophag ...
Prochlorococcus phage P-SSM2
Biochem. J.
433
469-476
2011
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3
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1
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1
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2
2
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724228
Busch
Structural and mechanistic ins ...
Prochlorococcus phage P-SSM2
Biochem. J.
439
257-264
2011
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4
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1
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1
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4
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3
3
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724317
Alvey
Attachment of noncognate chrom ...
Prochlorococcus phage P-SSM4
Biochemistry
50
4890-4902
2011
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1
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1
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10
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1
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1
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725252
Alvey
Effects of modified phycobilin ...
Synechococcus sp., Synechococcus sp. PCC 7335
J. Bacteriol.
193
1663-1671
2011
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1
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1
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1
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679363
Dammeyer
Efficient phage-mediated pigme ...
uncultured cyanophage
Curr. Biol.
18
442-448
2008
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6
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698811
Dammeyer
Phycoerythrobilin synthase (Pe ...
Prochlorococcus phage P-SSM2
J. Biol. Chem.
283
27547-27554
2008
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1
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7
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