Protein Variants | Comment | Organism |
---|---|---|
D105E | site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 | Prochlorococcus phage P-SSM2 |
D105N | site-directed mutagenesis, inactive mutant | Prochlorococcus phage P-SSM2 |
D206E | site-directed mutagenesis, the mutant catalyzes only the second reaction step, i.e. the formation of (3Z)-phycoerythrobilin from 15,16-dihydrobiliverdin, reaction of EC 1.3.7.3 | Prochlorococcus phage P-SSM2 |
D206N | site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 | Prochlorococcus phage P-SSM2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + 2 reduced ferredoxin | Prochlorococcus phage P-SSM2 | a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview | (3Z)-phycoerythrobilin + 2 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prochlorococcus phage P-SSM2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + 2 reduced ferredoxin | - |
Prochlorococcus phage P-SSM2 | (3Z)-phycoerythrobilin + 2 oxidized ferredoxin | - |
? | |
biliverdin IXalpha + 2 reduced ferredoxin | a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview | Prochlorococcus phage P-SSM2 | (3Z)-phycoerythrobilin + 2 oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PEB synthase | - |
Prochlorococcus phage P-SSM2 |
PebS | - |
Prochlorococcus phage P-SSM2 |
phycoerythrobilin synthase | - |
Prochlorococcus phage P-SSM2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Prochlorococcus phage P-SSM2 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the ferredoxin-dependent bilin reductase family | Prochlorococcus phage P-SSM2 |
metabolism | a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview | Prochlorococcus phage P-SSM2 |
additional information | aspartate residues Asp105 and Asp206 are both involved in interactions with the pyrrole nitrogens upon substrate binding. Both are essential for the complete reduction of biliverdin IXalpha to (3Z)-phycoerythrobilin by PebS and are highly conserved throughout the family of ferredoxin-dependent bilin reductases | Prochlorococcus phage P-SSM2 |