Application | Comment | Organism |
---|---|---|
additional information | conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa | Nostoc sp. |
Cloned (Comment) | Organism |
---|---|
wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha | Nostoc sp. |
Protein Variants | Comment | Organism |
---|---|---|
C210A | retains 90% relative to wild type | Nostoc sp. |
C86A | retains 90% activity relative to wild type | Nostoc sp. |
D102N | retains 11% activity relative to wild type | Nostoc sp. |
D116N | retains 11% activity relative to wild type | Nostoc sp. |
D217N | retains 87% relative to wild type | Nostoc sp. |
H71A | retains less than 1% activity relative to wild type | Nostoc sp. |
H71D | retains 28% activity relative to wild type | Nostoc sp. |
H71E | retains 50% activity relative to wild type | Nostoc sp. |
H71N | retains 46% activity relative to wild type | Nostoc sp. |
H71Q | retains 65% activity relative to wild type | Nostoc sp. |
H85A | retains less than 1% activity relative to wild type | Nostoc sp. |
H85D | retains less than 1% activity relative to wild type | Nostoc sp. |
H85E | retains 5% activity relative to wild type | Nostoc sp. |
H85N | retains less than 1% activity relative to wild type | Nostoc sp. |
H85Q | retains 5% activity relative to wild type | Nostoc sp. |
K218E | retains 20% activity relative to wild type | Nostoc sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethylpyrocarbonate | reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate | Nostoc sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. | - |
- |
- |
Nostoc sp. PCC 7120 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutants, histidine mutants purified to homogeneity | Nostoc sp. |
Storage Stability | Organism |
---|---|
-80°C | Nostoc sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXa + reduced ferredoxin | - |
Nostoc sp. | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
biliverdin IXa + reduced ferredoxin | - |
Nostoc sp. PCC 7120 | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PcyA | - |
Nostoc sp. |