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Literature summary for 1.3.7.5 extracted from

  • Tu, S.L.; Sughrue, W.; Britt, R.D.; Lagarias, J.C.
    A conserved histidine-aspartate pair is required for exovinyl reduction of biliverdin by a cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (2006), J. Biol. Chem., 281, 3127-3136.
    View publication on PubMed

Application

Application Comment Organism
additional information conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa Nostoc sp.

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha Nostoc sp.

Protein Variants

Protein Variants Comment Organism
C210A retains 90% relative to wild type Nostoc sp.
C86A retains 90% activity relative to wild type Nostoc sp.
D102N retains 11% activity relative to wild type Nostoc sp.
D116N retains 11% activity relative to wild type Nostoc sp.
D217N retains 87% relative to wild type Nostoc sp.
H71A retains less than 1% activity relative to wild type Nostoc sp.
H71D retains 28% activity relative to wild type Nostoc sp.
H71E retains 50% activity relative to wild type Nostoc sp.
H71N retains 46% activity relative to wild type Nostoc sp.
H71Q retains 65% activity relative to wild type Nostoc sp.
H85A retains less than 1% activity relative to wild type Nostoc sp.
H85D retains less than 1% activity relative to wild type Nostoc sp.
H85E retains 5% activity relative to wild type Nostoc sp.
H85N retains less than 1% activity relative to wild type Nostoc sp.
H85Q retains 5% activity relative to wild type Nostoc sp.
K218E retains 20% activity relative to wild type Nostoc sp.

Inhibitors

Inhibitors Comment Organism Structure
diethylpyrocarbonate reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
-
-
Nostoc sp. PCC 7120
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutants, histidine mutants purified to homogeneity Nostoc sp.

Storage Stability

Storage Stability Organism
-80°C Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXa + reduced ferredoxin
-
Nostoc sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
?
biliverdin IXa + reduced ferredoxin
-
Nostoc sp. PCC 7120 (3Z)-phycocyanobilin + oxidized ferredoxin
-
?

Synonyms

Synonyms Comment Organism
PcyA
-
Nostoc sp.