Protein Variants | Comment | Organism |
---|---|---|
E110Q | site-directed mutagenesis, the mutant shows 321.7% of wild-type activity | Arabidopsis thaliana |
E187Q | site-directed mutagenesis, the mutant shows 20.3% of wild-type activity | Arabidopsis thaliana |
H259Q | site-directed mutagenesis, the mutant shows 123.4% of wild-type activity | Arabidopsis thaliana |
K183Q | site-directed mutagenesis, the mutant shows 24.6% of wild-type activity | Arabidopsis thaliana |
K255Q | site-directed mutagenesis, the mutant shows 11.7% of wild-type activity | Arabidopsis thaliana |
K263Q | site-directed mutagenesis, the mutant shows 25.8% of wild-type activity | Arabidopsis thaliana |
R200Q | site-directed mutagenesis, the mutant shows 12.5% of wild-type activity | Arabidopsis thaliana |
R200Q/R264Q | site-directed mutagenesis, the mutant shows 11.9% of wild-type activity | Arabidopsis thaliana |
R264Q | site-directed mutagenesis, the mutant shows 18.9% of wild-type activity | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cofactor ferredoxin | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + reduced ferredoxin | Arabidopsis thaliana | - |
(3Z)-phytochromobilin + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SR43 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + reduced ferredoxin | - |
Arabidopsis thaliana | (3Z)-phytochromobilin + oxidized ferredoxin | - |
? | |
biliverdin IXalpha + reduced ferredoxin | conserved surface-charged residues on HY2 and Arabidopsis ferredoxin AtFd2 are important in the protein-protein interaction as well as biliverdin reduction activity of HY2, mutational analysis, overview. The C12 propionate group of biliverdin is important for HY2-catalyzed biliverdin reduction | Arabidopsis thaliana | (3Z)-phytochromobilin + oxidized ferredoxin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HY2 | - |
Arabidopsis thaliana |
phytochromobilin synthase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | one of the six Arabidopsis ferredoxins, AtFd2, is the preferred electron donor for HY2. Activity with ferredoxin AtFd2 mutants D78N, E81Q, E82Q, Y89F, R92Q, and D112N, overview | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | HY2 synthesizes the open chain tetrapyrrole chromophore for light-sensing phytochromes. It catalyzes the double bond reduction of a heme-derived tetrapyrrole intermediate biliverdin IXalpha at the A-ring diene system | Arabidopsis thaliana |