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Literature summary for 1.3.7.4 extracted from

  • Chiu, F.Y.; Chen, Y.R.; Tu, S.L.
    Electrostatic interaction of phytochromobilin synthase and ferredoxin for biosynthesis of phytochrome chromophore (2010), J. Biol. Chem., 285, 5056-5065.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E110Q site-directed mutagenesis, the mutant shows 321.7% of wild-type activity Arabidopsis thaliana
E187Q site-directed mutagenesis, the mutant shows 20.3% of wild-type activity Arabidopsis thaliana
H259Q site-directed mutagenesis, the mutant shows 123.4% of wild-type activity Arabidopsis thaliana
K183Q site-directed mutagenesis, the mutant shows 24.6% of wild-type activity Arabidopsis thaliana
K255Q site-directed mutagenesis, the mutant shows 11.7% of wild-type activity Arabidopsis thaliana
K263Q site-directed mutagenesis, the mutant shows 25.8% of wild-type activity Arabidopsis thaliana
R200Q site-directed mutagenesis, the mutant shows 12.5% of wild-type activity Arabidopsis thaliana
R200Q/R264Q site-directed mutagenesis, the mutant shows 11.9% of wild-type activity Arabidopsis thaliana
R264Q site-directed mutagenesis, the mutant shows 18.9% of wild-type activity Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ cofactor ferredoxin Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin IXalpha + reduced ferredoxin Arabidopsis thaliana
-
(3Z)-phytochromobilin + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SR43
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXalpha + reduced ferredoxin
-
Arabidopsis thaliana (3Z)-phytochromobilin + oxidized ferredoxin
-
?
biliverdin IXalpha + reduced ferredoxin conserved surface-charged residues on HY2 and Arabidopsis ferredoxin AtFd2 are important in the protein-protein interaction as well as biliverdin reduction activity of HY2, mutational analysis, overview. The C12 propionate group of biliverdin is important for HY2-catalyzed biliverdin reduction Arabidopsis thaliana (3Z)-phytochromobilin + oxidized ferredoxin
-
?

Synonyms

Synonyms Comment Organism
HY2
-
Arabidopsis thaliana
phytochromobilin synthase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
Ferredoxin one of the six Arabidopsis ferredoxins, AtFd2, is the preferred electron donor for HY2. Activity with ferredoxin AtFd2 mutants D78N, E81Q, E82Q, Y89F, R92Q, and D112N, overview Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function HY2 synthesizes the open chain tetrapyrrole chromophore for light-sensing phytochromes. It catalyzes the double bond reduction of a heme-derived tetrapyrrole intermediate biliverdin IXalpha at the A-ring diene system Arabidopsis thaliana