Cloned (Comment) | Organism |
---|---|
expressed using a tac promoter-driven N-terminal GST fusion protein | Synechococcus sp. |
into pGEX-6P-3 vector for N-terminal fusion with glutathione S-transferase and transformed in Escherichia coli BL21(lambdaDE3) cells | Microchaete diplosiphon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microchaete diplosiphon | Q6UR88 | - |
- |
Microchaete diplosiphon Fd33 | Q6UR88 | - |
- |
Synechococcus sp. | - |
- |
- |
Synechococcus sp. WH8020 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by two affinity chromatography steps to 90% purity and a third gel filtration step | Synechococcus sp. |
by two affinity chromatography steps to 90% purity and a third gel filtration step | Microchaete diplosiphon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon | phycoerythrobilin + oxidized ferredoxin | - |
r | |
15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon Fd33 | phycoerythrobilin + oxidized ferredoxin | - |
r | |
biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
biliverdin IXa + reduced ferredoxin | - |
Microchaete diplosiphon | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. WH8020 | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
biliverdin IXa + reduced ferredoxin | - |
Microchaete diplosiphon Fd33 | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
additional information | does not convert 3E-phycocyanobilin | Microchaete diplosiphon | ? | - |
? | |
additional information | does not convert phycocyanobilin | Synechococcus sp. | ? | - |
? | |
additional information | does not convert phycocyanobilin | Synechococcus sp. WH8020 | ? | - |
? | |
additional information | does not convert 3E-phycocyanobilin | Microchaete diplosiphon Fd33 | ? | - |
? | |
phytochromobilin + biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. | phycoerythrobilin | - |
r | |
phytochromobilin + biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. WH8020 | phycoerythrobilin | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PebA | - |
Synechococcus sp. |
PebA | - |
Microchaete diplosiphon |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Microchaete diplosiphon |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Synechococcus sp. | |
Ferredoxin | - |
Microchaete diplosiphon | |
additional information | insensitivity toward metal chelators like 0.01 mM EDTA, 0.005 mM o-phenanthroline, or 0.005 mM 2,2'-dipyridyl | Microchaete diplosiphon | |
NADP+ | - |
Synechococcus sp. | |
NADP+ | - |
Microchaete diplosiphon |