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Literature summary for 1.3.7.15 extracted from

  • Kiesel, S.; Waetzlich, D.; Lange, C.; Reijerse, E.; Broecker, M.J.; Ruediger, W.; Lubitz, W.; Scheer, H.; Moser, J.; Jahn, D.
    Iron-sulfur cluster-dependent catalysis of chlorophyllide a oxidoreductase from Roseobacter denitrificans (2015), J. Biol. Chem., 290, 1141-1154.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Roseobacter denitrificans

Protein Variants

Protein Variants Comment Organism
C145A the mutant of subunit BchY is inactive Roseobacter denitrificans
C35A the mutant of subunit BchZ is inactive Roseobacter denitrificans
C35D the mutant of subunit BchZ is inactive Roseobacter denitrificans
C35D the mutant of subunit BchZ shows 60% of wild type activity Roseobacter denitrificans
C35D/S94C the mutant of subunit BchZ is inactive Roseobacter denitrificans
C35S the mutant of subunit BchZ is inactive Roseobacter denitrificans
C62A the mutant of subunit BchY is inactive Roseobacter denitrificans
C86A the mutant of subunit BchY shows 50% of wild type activity Roseobacter denitrificans
C87A the mutant of subunit BchY is inactive Roseobacter denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
Roseobacter denitrificans
56000
-
-
Roseobacter denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate Roseobacter denitrificans
-
3-acetyl-3-devinylchlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
?

Organism

Organism UniProt Comment Textmining
Roseobacter denitrificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-loaded chelating Sepharose column chromatography Roseobacter denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
Roseobacter denitrificans 3-acetyl-3-devinylchlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
?

Subunits

Subunits Comment Organism
? x * 53000 + x * 56000 + x * 53000, SDS-PAGE Roseobacter denitrificans

Synonyms

Synonyms Comment Organism
(BchY/BchZ)2 protein
-
Roseobacter denitrificans
BchX subunit Roseobacter denitrificans
BchY subunit Roseobacter denitrificans
BchZ subunit Roseobacter denitrificans
COR
-
Roseobacter denitrificans

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
Roseobacter denitrificans