Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.7.13 extracted from

  • Saunders, A.H.; Golbeck, J.H.; Bryant, D.A.
    Characterization of BciB a ferredoxin-dependent 8-vinyl-protochlorophyllide reductase from the green sulfur bacterium Chloroherpeton thalassium (2013), Biochemistry, 52, 8442-8451 .
    View publication on PubMed
Search for more literature for 1.3.7.13

Cloned(Commentary)

Cloned (Comment) Organism
gene bciB, cloning in Escherichia coli strain JM109, recombinant expression of C-terminally His6-tagged in Escherichia coli strain BL21(DE3). Plasmid pET28-CthaBciB is cotransformed into strain BL21(DE3) along with the plasmid pDB1282, which contains the genes iscS, iscU, iscA, hscA, hscB, and fdx for Fe/S-cluster assembly from Azotobacter vinelandii under the control of an arabinose-inducible promoter to produce Escherichia coli strain AHS-1 Chloroherpeton thalassium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Chloroherpeton thalassium
-
 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Chloroherpeton thalassium ATCC 35110
-
 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Chloroherpeton thalassium B3QYX9
-
-
Chloroherpeton thalassium ATCC 35110 B3QYX9
-
-

Purification (Commentary)

Purification (Comment) Organism
gene bciB, recombinant C-terminally His6-tagged from Escherichia coli strain AHS-1 under anaerobic conditions by cobalt affinity chromatography, ultrafiltration, and desalting gel filtration Chloroherpeton thalassium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
-
 Chloroherpeton thalassium protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+
-
 Chloroherpeton thalassium ATCC 35110 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
additional information using electrons provided by reduced ferredoxin or dithionite, recombinant BciB is active and reduced the 8-vinyl moiety of the substrate, 8-vinyl protochlorophyllide, producing protochlorophyllide a. BciB requires an additional electron donor, ferredoxin, together with NADPH Chloroherpeton thalassium ?
-
 ?
additional information using electrons provided by reduced ferredoxin or dithionite, recombinant BciB is active and reduced the 8-vinyl moiety of the substrate, 8-vinyl protochlorophyllide, producing protochlorophyllide a. BciB requires an additional electron donor, ferredoxin, together with NADPH Chloroherpeton thalassium ATCC 35110 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, recombinant His6-tagged enzyme, SDS-PAGE Chloroherpeton thalassium

Synonyms

Synonyms Comment Organism
8-vinyl reductase
-
 Chloroherpeton thalassium
bciB
-
 Chloroherpeton thalassium
Ctha_1208
-
 Chloroherpeton thalassium
PChlide reductase
-
 Chloroherpeton thalassium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Chloroherpeton thalassium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Chloroherpeton thalassium

Cofactor

Cofactor Comment Organism Structure
FAD recombinant BciB binds a flavin adenine dinucleotide cofactor Chloroherpeton thalassium
additional information BciB requires an additional electron donor, ferredoxin, together with NADPH Chloroherpeton thalassium
NADPH
-
 Chloroherpeton thalassium
[4Fe-4S] cluster recombinant BciB binds two [4Fe-4S] clusters, one of which may not be essential for the activity of the enzyme, determined by EPR spectroscopy as well as quantitative analyses of bound iron and sulfide Chloroherpeton thalassium

General Information

General Information Comment Organism
additional information structural modeling of BciB based on a structure for the FrhB subunit of F420-reducing [NiFe]-hydrogenase of Methanothermobacter marburgensis, overview Chloroherpeton thalassium