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Literature summary for 1.3.7.13 extracted from

  • Canniffe, D.P.; Chidgey, J.W.; Hunter, C.N.
    Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis (2014), Biochem. J., 462, 433-440 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.7.13

Protein Variants

Protein Variants Comment Organism
additional information generation of an enzyme BciB knockout mutant and recombinant expression of Rhodobacter sphaeroides gene bciA in the mutant Synechocystis sp. PCC 6803

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Synechocystis sp. PCC 6803 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Synechocystis sp. PCC 6803 preferred substrate chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ Synechocystis sp. PCC 6803 is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
additional information Synechocystis sp. PCC 6803 although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions ?
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P74473
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ preferred substrate Synechocystis sp. PCC 6803 chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Synechocystis sp. PCC 6803 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
 ?
additional information although the native BciB is able to reduce the 8V group of Pchlide, the preferred substrate for this enzyme is 8V Chlide, with only a small amount of 8E Pchlide detected in the wild-type when grown under pigment accumulating conditions Synechocystis sp. PCC 6803 ?
-
 ?

Synonyms

Synonyms Comment Organism
8V reductase
-
 Synechocystis sp. PCC 6803
8VR
-
 Synechocystis sp. PCC 6803
bciB
-
 Synechocystis sp. PCC 6803
C8-vinyl reductase
-
 Synechocystis sp. PCC 6803
nucleoside-diphosphate-sugar epimerase UniProt Synechocystis sp. PCC 6803
RSP_3070
-
 Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
ferredoxin [iron-sulfur] cluster
-
 Synechocystis sp. PCC 6803
NADPH
-
 Synechocystis sp. PCC 6803

General Information

General Information Comment Organism
evolution two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB Synechocystis sp. PCC 6803
metabolism the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment Synechocystis sp. PCC 6803
additional information BciB functions as an 8VR and does not require the presence of an additional subunit Synechocystis sp. PCC 6803