BRENDA - Enzyme Database show
show all sequences of 1.3.5.5

Characterization of a higher plant herbicide-resistant phytoene desaturase and its use as a selectable marker

Arias, R.S.; Dayan, F.E., Michel, A.; Howell, J.; Scheffler, B.E.; Plant Biotechnol. J. 4, 263-273 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
agriculture
the Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Hydrilla verticillata
Engineering
Amino acid exchange
Commentary
Organism
R304X
substitution of arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Inhibitors
Inhibitors
Commentary
Organism
Structure
fluridone
arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
norflurazon
arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hydrilla verticillata
-
L. f. Royle
-
Application (protein specific)
Application
Commentary
Organism
agriculture
the Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Hydrilla verticillata
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R304X
substitution of arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fluridone
arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
norflurazon
arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
Hydrilla verticillata
Other publictions for EC 1.3.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2016
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743580
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Phytoene desaturase from Oryz ...
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10
e0131717
2015
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742721
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Dissection of tomato lycopene ...
Solanum lycopersicum
Plant Physiol.
163
986-998
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712989
Cong
cDNA cloning and expression an ...
Triticum aestivum
Mol. Biol. Rep.
37
3351-3361
2010
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713219
Lopez
Phytoene desaturase is present ...
Brassica oleracea
Physiol. Plant.
133
190-198
2008
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711700
Qin
Disruption of phytoene desatur ...
Arabidopsis thaliana
Cell Res.
17
471-482
2007
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710925
Steinbrenner
Transformation of the green al ...
Haematococcus lacustris
Appl. Environ. Microbiol.
72
7477-7484
2006
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713245
Arias
Characterization of a higher p ...
Hydrilla verticillata
Plant Biotechnol. J.
4
263-273
2006
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660319
Breitenbach
zeta-Carotene cis isomers as p ...
Capsicum annuum
Planta
220
785-793
2005
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712212
Zhu
Isolation and characterization ...
Dunaliella salina
J. Agric. Food Chem.
53
5593-5597
2005
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659604
Matthews
Maize phytoene desaturase and ...
Zea mays
J. Exp. Bot.
54
2215-2230
2003
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712211
Ohki
Phytoene desaturase inhibition ...
Synechococcus elongatus PCC 7942
J. Agric. Food Chem.
51
3049-3055
2003
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713208
Bennoun
The present model for chlorore ...
Embryophyta, Rhodophyta
Photosyn. Res.
73
273-277
2002
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711506
Kita
Cloning and expression of CitP ...
Citrus unshiu
Biosci. Biotechnol. Biochem.
65
1424-1428
2001
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712209
Sandmann
In vitro inhibition studies of ...
Synechococcus sp.
J. Agric. Food Chem.
49
138-141
2001
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712210
Breitenbach
Bleaching herbicide norflurazo ...
Gentiana lutea, Synechococcus sp.
J. Agric. Food Chem.
49
5270-5272
2001
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713304
Grünewald
Phytoene desaturase is localiz ...
Haematococcus lacustris
Plant Physiol.
122
1261-1268
2000
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713292
Wetzel
Regulation of phytoene desatur ...
Arabidopsis thaliana
Plant Mol. Biol.
37
1045-1053
1998
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713288
Al-Babili
A novel, soluble form of phyto ...
Narcissus pseudonarcissus
Plant J.
9
601-612
1996
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