BRENDA - Enzyme Database show
show all sequences of 1.3.5.5

Phytoene desaturase is present in a large protein complex in the plastid membrane

Lopez, A.B.; Yang, Y.; Thannhauser, T.W.; Li, L.; Physiol. Plant. 133, 190-198 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous expression in a strain of Escherichia coli containing a carotenoid-producing plasmid
Brassica oleracea
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the enzyme from stroma is in an approximately 660000 Da complex
Brassica oleracea
9507
-
chromoplast
BoPDS is present at similar levels in chromoplasts and leucoplasts
Brassica oleracea
9509
-
leucoplast
BoPDS is present at similar levels in chromoplasts and leucoplasts
Brassica oleracea
9516
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
350000
-
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
660000
-
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
15-cis-phytoene + acceptor
Brassica oleracea
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Brassica oleracea
B2X8P6
var. botrytis, white cultivar Stovepipe
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
the enzyme form from stroma appears to be slightly larger in size than the membrane version, which may be because of different posttranslational modifications of BoPDS in these two locations
Brassica oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue
Brassica oleracea
-
leaf
BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue
Brassica oleracea
-
root
siliques and roots show the lowest levels of expression
Brassica oleracea
-
seed
-
Brassica oleracea
-
silique
siliques and roots show the lowest levels of expression
Brassica oleracea
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-cis-phytoene + acceptor
-
713219
Brassica oleracea
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
15-cis-phytoene + acceptor
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
713219
Brassica oleracea
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous expression in a strain of Escherichia coli containing a carotenoid-producing plasmid
Brassica oleracea
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the enzyme from stroma is in an approximately 660000 Da complex
Brassica oleracea
9507
-
chromoplast
BoPDS is present at similar levels in chromoplasts and leucoplasts
Brassica oleracea
9509
-
leucoplast
BoPDS is present at similar levels in chromoplasts and leucoplasts
Brassica oleracea
9516
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
350000
-
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
660000
-
the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
15-cis-phytoene + acceptor
Brassica oleracea
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
the enzyme form from stroma appears to be slightly larger in size than the membrane version, which may be because of different posttranslational modifications of BoPDS in these two locations
Brassica oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue
Brassica oleracea
-
leaf
BoPDS has the highest level of expression in flowers with an approximate 4.5fold increase of BoPDS transcript level compared with leaf tissue
Brassica oleracea
-
root
siliques and roots show the lowest levels of expression
Brassica oleracea
-
seed
-
Brassica oleracea
-
silique
siliques and roots show the lowest levels of expression
Brassica oleracea
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-cis-phytoene + acceptor
-
713219
Brassica oleracea
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
15-cis-phytoene + acceptor
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
713219
Brassica oleracea
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55000, SDS-PAGE, the plastid membrane enzyme form is present in a large protein complex of approximately 350000 Da, the stroma version is in an approximately 660000 Da complex
Brassica oleracea
Other publictions for EC 1.3.5.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743898
Sandmann
Control of light-dependent ke ...
Nostoc sp. PCC 7120
Z. Naturforsch. C
71
303-311
2016
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1
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743580
Gemmecker
Phytoene desaturase from Oryz ...
Oryza sativa Indica Group
PLoS ONE
10
e0131717
2015
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742721
Alferez
Modification of carotenoid le ...
Citrus sinensis, Citrus sinensis L. Osbeck
J. Agric. Food Chem.
61
3082-3089
2013
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1
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743489
Fantini
Dissection of tomato lycopene ...
Solanum lycopersicum
Plant Physiol.
163
986-998
2013
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712989
Cong
cDNA cloning and expression an ...
Triticum aestivum
Mol. Biol. Rep.
37
3351-3361
2010
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713219
Lopez
Phytoene desaturase is present ...
Brassica oleracea
Physiol. Plant.
133
190-198
2008
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711700
Qin
Disruption of phytoene desatur ...
Arabidopsis thaliana
Cell Res.
17
471-482
2007
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710925
Steinbrenner
Transformation of the green al ...
Haematococcus lacustris
Appl. Environ. Microbiol.
72
7477-7484
2006
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713245
Arias
Characterization of a higher p ...
Hydrilla verticillata
Plant Biotechnol. J.
4
263-273
2006
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660319
Breitenbach
zeta-Carotene cis isomers as p ...
Capsicum annuum
Planta
220
785-793
2005
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712212
Zhu
Isolation and characterization ...
Dunaliella salina
J. Agric. Food Chem.
53
5593-5597
2005
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659604
Matthews
Maize phytoene desaturase and ...
Zea mays
J. Exp. Bot.
54
2215-2230
2003
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712211
Ohki
Phytoene desaturase inhibition ...
Synechococcus elongatus PCC 7942
J. Agric. Food Chem.
51
3049-3055
2003
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10
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713208
Bennoun
The present model for chlorore ...
Embryophyta, Rhodophyta
Photosyn. Res.
73
273-277
2002
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711506
Kita
Cloning and expression of CitP ...
Citrus unshiu
Biosci. Biotechnol. Biochem.
65
1424-1428
2001
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712209
Sandmann
In vitro inhibition studies of ...
Synechococcus sp.
J. Agric. Food Chem.
49
138-141
2001
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712210
Breitenbach
Bleaching herbicide norflurazo ...
Gentiana lutea, Synechococcus sp.
J. Agric. Food Chem.
49
5270-5272
2001
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713304
Grünewald
Phytoene desaturase is localiz ...
Haematococcus lacustris
Plant Physiol.
122
1261-1268
2000
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713292
Wetzel
Regulation of phytoene desatur ...
Arabidopsis thaliana
Plant Mol. Biol.
37
1045-1053
1998
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713288
Al-Babili
A novel, soluble form of phyto ...
Narcissus pseudonarcissus
Plant J.
9
601-612
1996
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